| Congresso Brasileiro de Microbiologia 2023 | Resumo: 1030-1 | ||||
Resumo:Candida albicans is a fungus that lives commensally in the human body and, in situations of imbalance in the immune system, it can cause superficial or invasive infections. This microorganism, responsible for more than 90% of invasive infections caused by fungi, possesses high pathogenicity due to its ability to metabolically adapt to the stress of the infectious process and its ability to form biofilms. Currently, due to the problems associated with the resistance of microorganisms to conventional treatments, the demand for new agents to control infections is increasing. Thus, the objective of the study was to evaluate the antimicrobial activity of nanoparticles alpha-silver tungstate (α-Ag2WO4) against suspensions of Candida albicans (ATCC 90028). The evaluated concentrations were 0.48, 0.97, 1.95 and 3.90 μg/mL. To determine the Minimum Inhibitory Concentration (MIC) and Minimum Fungicide Concentration (MFC) the microdilution method in Roswell Park Memorial Institute broth (RPMI – 1640) was used. C. albicans suspensions (2.5 x 103 CFU/mL) were incubated with the nanoparticles for 24 hours at 37ºC. Thereafter, to determine the MIC, the absorbance reading was carried out in a microplate reader (562 nm). CFM was obtained through the serial dilution process at a base 10 and plating using the microdrop method on Sabouraud Dextrose Agar (SDA), supplemented with chloramphenicol (5 μg/mL). The plates were incubated for 24 hours at 37°C and then, by counting the colonies, the amount of colony forming units per milliliter (CFU/mL) was established and, yielding the CFM. Assays were performed in triplicate, on three different occasions (n=9). The data obtained were analyzed using the Shapiro-Wilk test, for the analysis of normality, and then, the CFU/mL results were submitted to the Kruskal-Wallis test, followed by the Dunn’s post-test. For the results of the absorbance values, One-way analysis of variance (one-way ANOVA) test was applied followed by Tukey's post-test (α= 0.05). The MIC and CFM values of the nanoparticles were 3.90 μg/mL and 7.81 μg/mL, respectively. The reductions observed at concentrations of 0.48, 0.97, 1.95 and 3.90 μg/mL were 2.45, 2.25, 2.74 and 5.36 log10 (p<0.05), respectively. Therefore, it is concluded that α-Ag2WO4 nanoparticles are capable of inactivating C. albicans cells. Palavras-chave: Antimicrobial activity, Candida albicans, Nanoparticles, Silver tungstate Agência de fomento:FAPESP Nº: 13/07296-2 e 22/08487-5|CNPq Nº: 403985/2021-8 e 105473∕2022-7 | EUROPEAN UNION-NEXT GENERATION: MGS/2021/21 (UP2021-021). | |||||