| Congresso Brasileiro de Microbiologia 2023 | Resumo: 1027-1 | ||||
Resumo:The search for sustainable alternatives for the production of chemical and biotechnological compounds has become increasingly prominent. In this context, 2,3-butanediol has emerged as a valuable chemical product with diverse industrial applications, such as in the production of solvents, liquid fuel, and plastics. Bacillus licheniformis is an extensively studied bacterium capable of synthesizing 2,3-butanediol from renewable substrates. In this study, we investigated, for the first time, the production of 2,3-butanediol by B. licheniformis ATCC 14580 using cellulosic hydrolysate from eucalyptus as the carbon source. The cellulose pulp was obtained through kraft treatment of eucalyptus wood chips in a 20 L reactor, at 170 °C for 3 hours, using 8 L of cooking liquor with 22.5% active alkali and 25% sulfidity. Detailed analyses of the cellulose pulp and natural eucalyptus were performed using scanning electron microscopy (SEM) and X-ray diffraction (XRD), revealing significant structural modifications after the kraft treatment that facilitated the availability of cellulose during enzymatic hydrolysis by the enzyme Cellic CTec3. The cellulosic hydrolysate was used in fermentative assays with B. licheniformis, resulting in a production of 62.47 g/L of 2,3-butanediol from 100 g/L of glucose-rich hydrolysate, after 72-h at 45 °C and 180 rpm. The high yield of 0.62 g/g and productivity of 0.86 g/L/h in the production of 2,3-butanediol, a compound of added value, represent a significant advancement toward the utilization of renewable resources in the industry. This study demonstrated that cellulosic hydrolysate from eucalyptus treated by the kraft process is a promising source for 2,3-butanediol production by B. licheniformis ATCC 14580. Additionally, the detailed analysis of the eucalyptus structure and cellulosic pulp provided important insights into the modifications promoted by the kraft process. These results contribute to the development of more sustainable biotechnological processes, aiming to produce high-value compounds from renewable raw materials. Palavras-chave: Eucalyptus, Kraft treatment, Cellulosic pulp, Bacillus licheniformis, 2,3 butanediol Agência de fomento:CAPES (no. de processo 88887.479015/2020-00), FAPESP (no. de processo 2022/05214-8) e CNPq (no. de processo 309214/2021-1) | |||||