| Congresso Brasileiro de Microbiologia 2023 | Resumo: 1008-1 | ||||
Resumo:The Yaque del Norte River is one of the most critical water resources in the Dominican Republic, and its water quality has deteriorated due to wastewater from nearby urban and agricultural areas being discharged into the river. Enterobacteriaceae are found in the animal intestinal microbiota and are responsible for a quarter and a third of human bacterial diseases. The current situation of the river makes it a medium for the proliferation of antibiotic-resistant enterobacteria. The Yaque del Norte River is one of the most important in the Dominican Republic, the longest river in the territory and the second on the entire island. Throughout its 296 km route, it provides water to many cities and towns in the western and central areas of the Cibao region, such as Jarabacoa, Santiago, Mao, among others. Its waters are routinely used to feed the irrigation canals of the territories dedicated to agriculture on the northwest line. This research aimed to determine the existence of these bacteria and characterize their resistomes through high-throughput sequencing and bioinformatic analysis. Four bacterial strains were isolated using chromogenic culture media, one corresponding to each of the four sampling points around the river. A strain of K. pneumoniae and E. coli were identified in Jarabacoa, a strain of K. pneumoniae in Guayubín, and a strain of Enterobacter sp. in Monte Cristi. High-throughput technologies sequenced them, the genomes of these strains were reassembled and annotated, and all were found to have resistance to multiple antibiotics and to be pathogenic for humans. The K. pneumonia strain isolated from point A in Jarabacoa presented 14 resistance genes. Its resistome confers resistance to fluoroquinolones, fosfomycin, beta-lactams, trimethoprim, sulfonamide, and aminoglycosides. The E. coli strain isolated from point B in Jarabacoa presented 31 resistance genes. Its resistome confers resistance to fluoroquinolones, fosfomycin, beta-lactams, trimethoprim, sulfonamide, aminoglycosides, phenicols, macrolides, lincosamides, tetracycline, and nitroimidazoles. The K. pneumonia strain isolated from point C in Guayubín presented 20 resistance genes. Its resistome confers resistance to fluoroquinolones, fosfomycin, beta-lactams, aminoglycosides, nitroimidazoles, and streptothricin. The E. cloacae strain isolated from point D in Monte Cristi presented 13 resistance genes. Its resistome confers resistance to fluoroquinolones, fosfomycin, beta-lactams, aminoglycosides, nitroimidazoles, and streptothricin. The WHO warned of a future health crisis caused by antibiotic-resistant bacteria and published a list of bacteria for which new antibiotics must be developed, including carbapenem-resistant Enterobacteriaceae, such as the strains in this research. Palavras-chave: resistance genes, Enterobacteriaceae, Yaque del Norte river, Dominican Republic, resistomes Agência de fomento: Fondo Nacional de Innovación y Desarrollo Científico y Tecnológico-FONDOCYT | |||||