| Congresso Brasileiro de Microbiologia 2023 | Resumo: 1001-1 | ||||
Resumo:Salmonella spp. is a significant and widespread foodborne pathogen that poses a considerable threat to public health globally. It can enter the food chain at various stages and contaminate retail meat products, leading to potential risks when the meat is consumed undercooked or when it comes into contact with other foods in the kitchen. Retail markets play a crucial role in the distribution and sale of raw meat, making them potential sources of Salmonella contamination throughout the food supply chain. Therefore, this study aimed to investigate the prevalence and molecular characterization of Salmonella in raw meat available in retail markets in Campo Grande, State of Mato Grosso do Sul, Brazil. To conduct this study, 53 samples of approximately 100g of ground beef were collected from retail markets, covering all regions of Campo Grande, MS. For isolation of Salmonella spp. from the samples, initially, 25g of each sample was enriched with 225 ml of Buffered Peptone Water, followed by homogenization for 120 seconds and incubation at 37 °C for 24 hours. Next, a 100 µl aliquot of the pre-enriched inoculum was transferred to 10 ml of Rappaport-Vassiliadis broth and Muller-Kauffmann Tetrathionate Novobiocin Broth and incubated at 42 °C for 24 hours. Colonies suspected to be Salmonella were then streaked onto Xylose-Lysine-Desoxycholate agar and Salmonella-Shigella agar. After a 24-hour incubation period at 37 °C, the presumptive colonies were subjected to further confirmation through biochemical tests on Triple Sugar Iron agar, urease, Lysine Iron Agar, Sulphide Indole Motility medium, and citrate, as well as PCR targeting invA and amplicon sequencing. Out of the 53 samples analyzed, 15 (28.3%) presented a biochemical profile compatible with Salmonella spp., and 6 (11.3%) tested positive for Salmonella invA gene through PCR, which was further confirmed by amplicon sequencing. In conclusion, this study sheds light on the prevalence of Salmonella in raw meat available in retail markets in Campo Grande, MS, Brazil. The findings highlight the importance of monitoring and implementing effective control measures to reduce Salmonella contamination in the food supply chain and safeguard public health. Palavras-chave: Salmonella spp., ground meat, retail market, PCR, biochemical tests Agźncia de fomento:Universidade Federal de Mato Grosso do Sul; CNPq; Universidade Católica Dom Bosco. | |||||