| Congresso Brasileiro de Microbiologia 2023 | Resumo: 979-1 | ||||
Resumo:In veterinary medicine, especially in small animals, there is not much data regarding vancomycin resistance of Enterococcus (VRE), but multidrug-resistant Enterococcus are regularly identified, favoring the spread of multidrug-resistant microorganisms to humans due to their zoonotic potential. The objective of this study was to determine the presence of the major resistance genes to vancomycin colonizing cats and dogs hospitalized in a veterinary hospital. A total of 59 VRE, being 48 E. faecalis (VREfls), 6 E. gallinarum (VREgll) group and 5 E. faecium (VREfcm), isolated from cats and dogs were submitted a Multiplex PCR, for the detection of vancomycin genes (vanA, vanB, vanC, vanD, vanE and vanG). For identification of vancomycin resistance genes the protocol of multiplex PCR was followed: 50 μL final volume containing PCR buffer (Tris-HCl - 20mM, KCl - 50mM), MgCl2 (1.5mM), dNTP (200mM of each), Taq DNA polymerase (1U), primers (4 pmol of each), and bacterial DNA (3µL). The program was carried out as follows: initial denaturation at 94ºC for 10 min; 40 cycles of denaturation step at 94 °C for 40 s, annealing of the primer at 56 °C for 40 s, and 1 min of extension at 72 °C. The last cycle extension was at 72 °C for 7 min. Enterococcus faecium (ATCC 51299) and Enterococcus faecalis (ATCC 700221) reference strains were used to assure quality control for the detection. Escherichia coli ATCC 25922 reference strain was used as a negative control. Enterococcus was identified with the presence of the vanA gene (52,54% - 45,76% VREfls and 6,78% VREfcm), vanB gene (23,73% - 22,03 VREfls and 1,7% VREfcm), vanC (20,34% - 10,17% VREfls and 10,17% VREgll) and vanE gene (3,39% VREfls). The vanD and vanG genes were not detected. Despite having been described in other works, the vanE genes in E. faecalis, the vanB gene in E. faecium and vanC gene in E. faecalis are not common in human and animals, much less in dogs and cats, as isolated in some animals in this study, which shows the importance of detecting these genes in animals that are getting closer and closer to us humans. The animals that harbor these genes serve as reservoirs for possible contamination of other animals, humans and the environment. The non-existence of a Hospital Infection Control Commission at a veterinary hospital in Brazil increases the chance of dissemination of multiresistant microorganisms in the environment, which contributes to the contamination of hospitalized animals, further aggravating the health status of the animal and exposing health professionals contact with these microorganisms. For the authors, this is the first report demonstrating the presence of the vanE gene in a dog in Brazil. Palavras-chave: VRE, small animals, multidrog resistance, vanE, veterinary Agência de fomento:Fapesc | |||||