| Congresso Brasileiro de Microbiologia 2023 | Resumo: 971-1 | ||||
Resumo:The prevalence of carbapenemase production, although these are widespread in different countries, varies according to the regions of the world. VIM, NDM, KPC, OXA-48 and IMP carbapenemases are the most commonly found in health centers, thus limiting therapeutic options. This study aimed to determine the molecular epidemiology of carbapenamases in gram-negative bacilli (gnb) isolated from patients admitted a teaching hospital in the northern region of ceará. from february and june 2023, 12 GNB producers of carbapenemases from cultures of different biological sites of patients hospitalized in the wards and Intensive Care Units of Hospital Santa Casa de Misericórdia de Sobral (SCMS) – Ce were analysed. All isolates were identified in the SCMS microbiology laboratory using the VITEK®2 automated system. Afterwards, the specimens were sent to the Central Laboratory of Ceará (LACEN) in Fortaleza - Ce, where the Xpert ® Carba-R tests were performed by real-time multiplex PCR to detect the genetic sequences of bla KPC, bla NDM, bla VIM, bla OXA-48, and bla IMP. Of the total sites analyzed, it was observed that urine (n = 6; 50%) was the most frequent site of isolation, followed by tracheal aspirate (n = 3; 25%) and blood (n = 3; 25%). In addition, most GNB were isolated from Intensive Care Units (n = 5; 42%) and Neurology (n = 4; 33%), followed by Surgical Clinic (n = 1; 8%), Internal Medicine (n = 1; 8%) and Pediatric ICU (n = 1; 8%). Carbapenemase-producing microorganisms detected during the analyzed period were Acinetobacter baumanni (n=3; 25%), Klebsiella pneumoniae (n=3; 25%), Entobacter aerogenes (n=3; 25%), Proteus mirabilis (n=2; 17%), and Pseudomonas aeruginosa (n = 1; 8%). Regarding the resistance genes analyzed, only the three K. pneumoniae isolates harbored the bla KPC gene. However, 3 (25%) microorganisms carried the bla NDM gene, being E. aerogenes (n = 2) and K. pneumoniae (n = 1). On the other hand, the bla VIM, bla OXA-48 and bla IMP genes were not found in any analyzed BGN. Therefore, data from this study revealed a predominance of KPC and NDM-type carbapenemases among the GNB isolated from SCMS. This fact corroborates, at the local level, the worrying dissemination both nationally and globally of these β-lactamases, as well as the predisposition of bacteria that carry these genes to cause healthcare-associated infections, in view of their abilities to accumulate and transfer resistance mechanisms. Palavras-chave: Antimicrobial resistance, bla KPC, bla NDM, carbapenemase, healthcare-associated infection Agência de fomento:Santa Casa de Misericórdia de Sobral | |||||