Congresso Brasileiro de Microbiologia 2023

Congresso Brasileiro de Microbiologia 2023

Resumo: 961-2

961-2

RESEARCH ON CLASS I INTEGRONS IN Escherichia coli, Salmonella sp., Enterobacteriaceae and Pseudomonas sp ISOLATED IN EFFLUENTS FROM PIG, CATTLE AND POULTRY SLAUGHTERHOUSES

Autores:

Patrícia Regina Lopes Melo (UNESP - SÃO PAULO STATE UNIVERSITY ) ; Evelyn Cristine da Silva (UNESP - SÃO PAULO STATE UNIVERSITY ) ; Emanoelli Aparecida Rodrigues dos Santos (UNESP - SÃO PAULO STATE UNIVERSITY ) ; Leonardo Ereno Tadielo (UNESP - SÃO PAULO STATE UNIVERSITY ) ; Gabriella Moura Lopes Simião (UNESP - SÃO PAULO STATE UNIVERSITY ) ; Linley Viana Maciel (UNESP - SÃO PAULO STATE UNIVERSITY ) ; Gean Carlo Azinari (UNESP - SÃO PAULO STATE UNIVERSITY ) ; Ricardo Seiti Yamatogi (UFV - UNIVERSIDADE FEDERAL DE VIÇOSA, UFL - EMERGING PATHOGENS INSTITUTE, UNIVERSITY OF FLORIDA) ; João Pessoa Araújo Junior (UNESP - SÃO PAULO STATE UNIVERSITY ) ; Fábio Sossai Possebon (UNESP - SÃO PAULO STATE UNIVERSITY ) ; Juliano Gonçalves Pereira (UNESP - SÃO PAULO STATE UNIVERSITY )

Resumo:

Integrons are structures that enable the expression and exchange of genes containing antibiotic resistance determinants. These genes can be acquired from other bacteria or through genetic recombination, leading to rapid dissemination of resistance within bacterial groups. The objective of this study was to assess the presence of class 1 integrons in Escherichia coli, Salmonella sp., Enterobacteriaceae, and Pseudomonas sp. isolated from effluents of swine, cattle, and poultry slaughterhouses using the PCR technique. Three effluent samples were collected after final treatment at slaughterhouses accredited by federal and state inspection services in the state of São Paulo, Brazil. The presence of E. coli, Salmonella sp., Enterobacteriaceae (EB), and Pseudomonas sp. was evaluated according to the standard protocol described by the International Organization for Standardization. After isolation and biochemical characterization, the isolates were characterized through PCR for the presence of the int1 gene. Thirteen cultures of E. coli, one culture of Salmonella sp., sixteen cultures of EB, and five cultures of Pseudomonas sp. were obtained. The presence of the int1 gene was detected in six isolates of E. coli (46.1%), twelve isolates of EB (75%), the isolate of Salmonella sp., and three isolates of Pseudomonas sp. (60%). Our results demonstrate a high presence of genes associated with bacterial resistance in a widely distributed microbial group in the environment. In an effort to reduce environmental and public health risks, various treatment methods are employed for effluents generated during the technological slaughter process. However, the processes used do not appear to eliminate the presence of pathogenic microorganisms, thus exposing the environment to resistance genes, which may have a potential impact on public health since these effluents are returned to the environment after treatment. DEVELOPMENT AGENCY: Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Process nº 2023/01185-6; Process nº 2022/03062-6. Process nº 2023/00969-3.

Palavras-chave:

INTEGRON, EFFLUENTS, PCR

Agência de fomento:

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)