| Congresso Brasileiro de Microbiologia 2023 | Resumo: 961-1 | ||||
Resumo:The Enterobacteriaceae family constitutes a group of Gram-negative microorganisms that includes species of medical and environmental significance. A concerning aspect is the potential dissemination of antibiotic resistance within this bacterial group. Therefore, the objective of this study was to evaluate the antibiotic resistance phenotypic profile and the presence of class 1 integrons on Enterobacteriaceae isolates obtained from surface samples of food processing industries after operational hygiene procedures. A total of 480 samples were collected from a poultry slaughterhouse (n=160), a swine slaughterhouse (n=160), and a dairy facility (n=160), all regularly inspected by the Federal Inspection Service. Enterobacteriaceae isolation was performed on Violet Red Bile Glucose Agar (VRBG), and colonies with typical morphology were confirmed using the oxidase test. The confirmed isolates were evaluated using the disk diffusion method on agar, following the guidelines of the Clinical and Laboratory Standards Institute (CLSI), for the following antimicrobials: amoxicillin - AMO (10 μg), imipenem - IPM (10 μg), cefotaxime - CTX (30 μg), ceftazidime - CAZ (30 μg), chloramphenicol - CLO (30 μg), ciprofloxacin - CIP (5 μg), nalidixic acid - NAL (30 μg), gentamicin - GEN (10 μg), and tetracycline - TET (30 μg). Isolates resistant to more than three antimicrobial classes were classified as multidrug-resistant (MDR). A total of 150 isolates were obtained from the surface samples. Among these isolates (n=150), 113 (75.3%) were resistant to at least one of the evaluated antibiotics, and only 3 (2%) were MDR. The highest percentages of antibiotic resistance were observed for AMO (61.3%), IPM (24.6%), and CTX (7.3%). No isolates showed resistance to gentamicin, chloramphenicol, or ciprofloxacin. The presence of the int1 gene was detected in 23 isolates (15.3%), including one MDR isolate, 14 isolates showing resistance to at least one class of antibiotics, and 8 isolates without resistance to any class evaluated in the phenotypic test. The isolates evaluated in this study did not demonstrate a high percentage of MDR. However, a high resistance to amoxicillin, a key antibiotic for Gram-negative bacteria, was observed. Hence, the low frequency of MDR does not exempt these microorganisms from posing a risk to public health. Mechanisms of resistance, such as horizontal gene transfer, can facilitate the dissemination and exchange of genetic factors among microorganisms, contributing to the increase or presence of antimicrobial resistance in previously non-MDR microorganisms.
DEVELOPMENT AGENCY: The authors thank the National Council for Scientific and Technological Development (Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq, Brasília, DF, Brazil), the Coordination for the Improvement of Higher Education Personnel (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES, Brasília, DF, Brazil – Code 001). Palavras-chave: ENTEROBACTERIA, ANTIMICROBIALS, RESISTANCE Agência de fomento:National Council for Scientific and Technological Development (Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq, Brasília, DF, Brazil) | |||||