| Congresso Brasileiro de Microbiologia 2023 | Resumo: 952-3 | ||||
Resumo:Calotropis procera (Aiton) Dryand is a laticifer that occurs mainly in arid/semi-arid regions. However, due to its high tolerance to abiotic stress conditions, it has become invasive in different biomes. Originally native to India, it is now present throughout almost all Brazilian territory. The use of C. procera in traditional medicine is well-established, and recent studies have confirmed its pharmacological potential. Despite this, little is known about the endophytic community of the plant, particularly in its latex. The aim of this study was to isolate and identify microorganisms from C. procera latex collected in Fortaleza - Ceará. Latex samples were coleted from three plants, located at Campus do Pici, Federal University of Ceará. The collected C. procera was sanitized with 70% ethanol, and the fresh latex was recovered in esterile 0.9% NaCl, then kept on ice until processing. Fresh latex was distributed in culture media using the spreader plate technique. Sabouraud Dextrose Agar and Plate Count Agar media were used and incubated at 30ºC and 35ºC, respectively, for four days. Different morphotypes were isolated and subcultured under the same conditions that originally showed growth. DNA extraction was performed using the technique of Cetyltrimethyl Ammonium Bromide (CTAB 2X), and the concentration and quality of all DNA were verified using Nanodrop spectrophotometer. The 16S bacterial ribossomal gene was amplified by PCR using the universal primer set 27F and 1525R and sequenced with the primers 518F and 800R. For fungi the ITS gene was amplified and sequenced using the primer set ITS1 and ITS4. The amplified PCR products were sequenced using the automated sequencer SeqStudio. The obtained sequences were analyzed and aligned using Geneious Prime, and, the taxonomic classification was performed through the Basic Local Alignment Search Tool (BLAST). The identified bacterial strains included Brevibacterium sp. (100% similarity), Bacillus velezensis (100% similarity), Bacillus subtilis (100% similarity), and Bacillus licheniformis (99.5% similarity). Five yeasts identified as Rhodotorula mucilaginosa were isolated, all with 100% identity, except for the LT14 strain which showed similarity of 99.81%. No filamentous fungus grew under the conditions tested in the study, possibly due to the time interval used. The study demonstrated that the microbial community present in C. procera latex is diverse and can be cultivated by traditional methods. Endophytic microorganisms from medicinal plants are highly coveted by the pharmaceutical industry, as many of them are capable of producing the same bioactive substances as of the plants they colonize. Palavras-chave: community endophytic, laticiferous plant, microbial diversity, pharmacological potential Agência de fomento:FUNCAP, CNPq, CAPES | |||||