| Congresso Brasileiro de Microbiologia 2023 | Resumo: 951-1 | ||||
Resumo:Candida albicans can infect root canals being mostly isolated from persistent endodontic infections. Adhesion to dentin is a first step for a successful microbial root canal colonization. The cell hydrophobicity seems to have some influence in the Candida species adhesion to surfaces. Hence, this in vitro study aimed to measure the cell surface hydrophobicity and to investigate the adherence ability to human dentin among Candida albicans strains isolated from root canal and lingual dorsum. Adhesion was quantified in function of dentin area covered by blastospores and/or hyphae using a confocal microscope. The cell surface hydrophobicity (CSH) was measured by a biphasic aqueous-hydrocarbon assay. Statistical analysis was performed by using GraphPad Prism software version 8. Differences in relative CSH value, and the dentin adhesion area between the Candida groups were tested using Kruskal–Wallis test. Spearman’s rank correlation was used to test the correlation between CSH value and adhesion ability. Statistically significant difference was considered with a p-value <0.05. Regardless some intra-species variation in cell surface hydrophobicity, the most of isolates was characterized by a moderate hydrophobicity. Lingual dorsum isolates showed a mean relative hydrophobicity value of 35.99% with a range of 3.66-67.67%. Among yeasts previously isolated from the root canals, the hydrophobicity mean was 31.61% ranging from 6.44 to 56.33%. There was no significant difference in this parameter when the isolation niche was considered. Both root canal and lingual dorsum yeasts were able to adhere to dentin. No association was found between strains site of isolation and adhesion. On correlating the results of adhesion to dentin surface and the relative CSH for lingual dorsum C. albicans, it was not found a significant positive correlation between these two attributes (r= 0.1429, p= 0.8028). Among root canal isolates, it was observed a negative correlation between hydrophobicity and adhesion but there was no significance (r= -0.7714, p=0.1028). Although, hydrophobicity can influence Candida albicans virulence in many ways, this study concluded that this parameter by itself was not a good predictor of adhesion to dentin. Palavras-chave: Candida albicans, dentin adhesion, endodontic infection, hydrophobicity Agência de fomento:CNPq e FAPEMIG | |||||