| Congresso Brasileiro de Microbiologia 2023 | Resumo: 931-2 | ||||
Resumo:Many anuran amphibians deposit their eggs in foam nests, which are biostructures composed mostly by proteins, that help protect the eggs and tadpoles from predators and environmental stress. Currently, there are no other identification and description studies of the cultivable microbiota and its role in the nests of the Leptodactylid frogs such as Physalaemus cuvieri, Leptodactylus vastus and Adenomera hylaedactyla. This study, aimed to isolate and identify the culturable bacteria from these three anuran species' nests, as well as to prospect enzymes produced by this microbiota. Foam nests samples and environmental samples from these three species were diluted and viable cell count was determined. Bacterial morphotypes from foam nest samples were isolated through the spread plate technique. Isolates’ DNAs were extracted followed by rRNA 16S gene amplification and Sanger sequencing. To evaluate their enzymatic potential, the isolates were cultured in ATGE medium supplemented with starch (0.1% w/v), gelatin (3% w/v) and skimmed milk (1% w/v), to verify amylase and protease activity. A total of 183 bacterial morphotypes were isolated, comprising 33 bacterial genera. Proteobacteria phylum was the most abundant in all the three nests (79%). The genera Pseudomonas and Aeromonas were the most abundant taxon in Physalaemus cuvieri and Leptodactylus vastus nests. On the other hand, in Adenomera hylaedactyla, the most abundant taxon were Enterobacter and Bacillus. Regarding the enzymatic activities, 130 isolates displayed protease activity and 45 isolates were positive for amylase activity. Our results provide unprecedented information concerning culturable bacterial microbiota of the foam nests of the Leptodactylid frogs, as well as their potential for biomolecules of biotechnological interest. Palavras-chave: Microbiome, Amphibia, Leptodactylus, Physalaemus, Adenomera Agência de fomento:CNPq; FUNCAP; CAPES | |||||