| Congresso Brasileiro de Microbiologia 2023 | Resumo: 931-1 | ||||
Resumo:Anurans are the vertebrates that suffer the most population decline and have the highest extinction rates due to chytridiomycosis, an emerging disease caused by the pathogenic fungus Batrachochytrium dendrobatidis (Bd). Bacteria with anti-Bd activity present in the cutaneous microbiota of these animals can play an important role in the control of chytridiomycosis in affected individuals, becoming candidates for the development of probiotics that can help strengthen the defenses of these animals against the pathogen. Another source of anti-Bd bacteria that has not yet been explored is foam nests, that are the reproductive mode of some frog species that harbor a unique microbiome, which may also contribute to the development of probiotics that will act from the early stages of development of these animals. Thus, this research aimed to prospect anti-Bd bacteria in foam nests of the frogs Adenomera hylaedactyla, Leptodactylus vastus and Physalaemus cuvieri, aiming to obtain a probiotic isolate or consortium aimed at controlling the pathogen. Three microbial collections from foam nests of the mentioned anuran species were used, totaling 182 bacterial isolates. The evaluation of the bacteria's anti-Bd potential was carried out using two methods: in solid medium (Trypton Agar 1%), and in microculture in liquid medium (Trypton Broth 1%), containing Bd culture and cell-free supernatants from co-cultures of the isolates with the fungus. As a result, in the test in solid medium, 31% of the samples showed inhibition of the fungus by halo formation, while in the test in liquid medium, 5% of high inhibition, 8% of moderate inhibition, 3% of low inhibition, 19 % no effect and 65% facilitation. In both tests, the genus with greater prominence for anti-Bd activity was Pseudomonas. Based on the results of the two tests, 12 candidate isolates were selected to compose an anti-Bd probiotic. A cross streak methodology was used to verify the compatibility of the microorganisms, in which one isolate showed inhibition against all the others. Thus, 11 isolates were classified as candidate targets for the assembly step of possible microbial consortia to act in the control of chytridiomycosis. Palavras-chave: Microbiota, Nidobiome, Chytridiomycosis, Probiotics, Microbial consortium Agência de fomento:CNPq; FUNCAP; CAPES | |||||