| Congresso Brasileiro de Microbiologia 2023 | Resumo: 929-1 | ||||
Resumo:Mimosa tenuiflora (Jurema preta) is a pioneer plant found in areas undergoing desertification in the Caatinga. M. tenuiflora plays an important role in the reconstruction of vegetation in the desertification area in Irauçuba, Ceará, but the abundance and diversity of its endophytes have not yet been investigated. Plant endophytic bacteria can play an important role in resistance to stress such as desertification. Furthermore, many studies have shown that endophytic bacteria can promote plant growth and increase absorption of mineral elements by the host to increase its viability. The plants can develop unique endophytic communities at environmental extremes, such arid regions, which are highly valuable for research and application. This study was carried out to isolate and characterize plant growth-promoting bacteria (PGPB) among the endophyte community of M. tenuiflora with the aim of obtaining a consortium to accelerate vegetation restoration in degraded areas in Caatinga. For this purpose, samples of Jurema-preta seeds and roots from native areas, areas in the process of recovery (fallow) and areas in an advanced stage of desertification were collected. The samples were macerated in saline solution, serially diluted, and inoculated in plates containing media of different nutritional composition. The isolates were macro and microscopically characterized and tested to produce indole-acetic acid, ammonia, siderophores and exopolysaccharides, and to solubilize potassium and inorganic phosphate. In all, 90 isolates were obtained, four from seeds and 86 from plant roots. All isolates are Gram-positive bacteria. Among the nine most promising isolates, three were able to produce indole-3-acetic acid; four were ammonia producers; four were able to solubilize inorganic phosphate; three were able to solubilize potassium; one shows promise for the production of siderophores and one for exopolysaccharides. Two isolates stand out for being able to produce ammonia and for solubilizing P and K. All promising isolates were compatible with each other, which allows different combinations to produce customized consortia of PGPB. This study is the first report of PGPB associated with Jurema-preta, a pioneer plant in Caatinga, and represents a unique source for inoculums production or biofertilizers to help in the recovery of vegetation under severe risk of desertification. Palavras-chave: Biofertilizer, Environmental recovery, Mimosa tenuiflora, Plant endophytic bacteria, Root and seed microbiome Agência de fomento:CAPES; Funcap; CNPq | |||||