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Congresso Brasileiro de Microbiologia 2023
 		Resumo: 925-1

925-1

Investigation of the microbiota of peripheral venous catheters and its association with clinical outcome

Autores:
Gustavo Francisco Lopes (EERP-USP - Ribeirão Preto College of Nursing- University of São Paulo, HCFMRP-USP - Hospital das Clínicas of the Faculty of Medicine of Ribeirão) ; Viviane de Cássia Oliveira (FORP- USP - Ribeirão Preto School of Dentistry - University of São Paulo) ; Rachel Maciel Monteiro (EERP-USP - Ribeirão Preto College of Nursing- University of São Paulo) ; Pedro Castania Amadio Domingues (EERP-USP - Ribeirão Preto College of Nursing- University of São Paulo) ; Felipe Lazarini Bim (EERP-USP - Ribeirão Preto College of Nursing- University of São Paulo) ; Lucas Lazarini Bim (EERP-USP - Ribeirão Preto College of Nursing- University of São Paulo) ; Gabriela Bassi Ferreira Silva (EERP-USP - Ribeirão Preto College of Nursing- University of São Paulo) ; André Pereira Santos (EERP-USP - Ribeirão Preto College of Nursing- University of São Paulo) ; Cássio do Nascimento (FORP- USP - Ribeirão Preto School of Dentistry - University of São Paulo) ; Denise de Andrade (EERP-USP - Ribeirão Preto College of Nursing- University of São Paulo) ; Evandro Watanabe (EERP-USP - Ribeirão Preto College of Nursing- University of São Paulo, FORP- USP - Ribeirão Preto School of Dentistry - University of São Paulo)

Resumo:
Peripheral venous catheters ⟨ PVC ⟩ are medical devices commonly used in hospitalized patients to administrate intravenous therapy, and for hemodynamic monitoring. They can be associated with microbial contamination and require regular monitoring to prevent complications. This study evaluated the clinical occurrence of phlebitis and the associated-microbiological indicators. Initially, the prospective longitudinal follow-up of hospitalized patients on continuous use of flexible PVC and the frequency of signs and symptoms of injuries associated with catheterization were evaluated. In parallel, the assessment of bacterial contamination was carried out through culture-based methods, microscopy observation and 16S rRNA gene sequencing. Peripheral venous catheterization of 67 hospitalized patients were analyzed. The mean length of stay of the flexible PVC was 43 hours. As regards to the insertion sites, 98.5% were in the upper limbs: arms ⟨44.7% ⟩, hands ⟨35.8% ⟩ and forearms ⟨17.0% ⟩, and of these, 75.0% had a transparent dressing. Considering both methods for microbiological assessment, Staphylococcus spp. and Pseudomonas spp. were the most isolated bacterial genera on the PVC surface. Approximately one in four patients presented signs and symptoms of injuries associated with the use of flexible PVC; however, there was no association between clinical evaluation and the presence of microorganisms on the internal and external surfaces of the flexible PVC. Although there was no association between clinical and microbiological outcomes regarding the use of flexible PVC, it was observed pathogenic microorganisms and biofilm formation on the external and internal surface of the flexible PVC, indicating that it is a potential source of microorganisms associated with local and systemic infections.

Palavras-chave:
 Phlebitis, Biofilms, Catheters, Vascular Access Devices, 16S rRNA gene sequencing


Agência de fomento:
National Council for Scientific and Technological Development (CNPq) and Coordination for the Improvement of Higher Education Personnel (CAPES)