| Congresso Brasileiro de Microbiologia 2023 | Resumo: 916-1 | ||||
Resumo:Antimicrobial resistance threatens the effective prevention and treatment of an ever-increasing range of infections caused by bacteria. During COVID-19 pandemic, the incidence of bacterial infection in hospitalized COVID-19 patients was high, especially those caused by multidrug-resistant Gram-negative bacteria such as Pseudomonas aeruginosa, an emerging multidrug-resistant global opportunistic pathogen, more commonly associated with respiratory infections in humans. The aim of this study was to typing P. aeruginosa strains (n=18) isolated from hospitalized patients during COVID-19 pandemic in a hospital localized in the municipality of Rio de Janeiro/RJ, Brazil, from July/2021 to March/2022. The 18 strains were isolated from 17 patients (11 males and seven females): 12 with COVID-19 co-infection, four only with P. aeruginosa, and two found during Hospital Infection Prevention and Control (HIPC) monitoring; with ages varying from 45 to 97 years. Clinical specimens included tracheal secretion (n=9), urine (n=6), rectal swab (n=2), and oral swab (n=1). The strains were identified by semi-automatic VITEK®2 system and Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry using MALDI Biotyper® and were typing by Fourier-Transform Infrared Spectroscopy (FTIR). Antimicrobial susceptibility test (AST) was evaluated using AST-N409 cards by the VITEK®2 system according to BRCAST. Thirteen FTIR profiles were obtained with a cut-off of 0.261, a ratio of 1.54 strain/profile. From the three clusters formed, the one that compresences IR13 profile had only COVID-19 co-infections strains from the same local, indicating that they possibly had the same common source. Strains from the IR11 cluster were isolated from HIPC samples and other locals, indicating that this clone may be present in the hospital environment. No correlation could be inferred from the two strains of the IR8 profile. Nine (50.0%) strains were susceptible to all antibiotics tested. No association between the AST and FTIR profiles was observed since resistance and susceptible strains were found in the same cluster. Considering the necessity to use broad-spectrum antibiotics in cases of P. aeruginosa infections, the resistance found to four classes of antimicrobial agents (&beta-lactam, cephems, folate pathway antagonists, and fluoroquinolones) is worrisome. In conclusion, the FTIR seems to be an interesting tool for the evaluation of the clonality of P. aeruginosa and a helpful approach to understanding the epidemiology of this bacteria. Palavras-chave: Pseudomonas aeruginosa, FITR, antibiogram, typing, infection Agência de fomento:Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |||||