| Congresso Brasileiro de Microbiologia 2023 | Resumo: 874-1 | ||||
Resumo:Escherichia coli is a ubiquitous microorganism, colonizing both humans and animals and widely distributed in various compartments, making it a potential biomarker to study the spread of Antimicrobial Resistance (AMR). The aim of this research was to characterize E. coli isolates, obtained between September 2021 to May 2022, and evaluate the dynamics of their dissemination in the state of Paraíba, northeast of Brazil. Eleven bacterial isolates from raw milk were obtained from distinct dairy farms in the state and included in the study. Isolates from clinical source were obtained from patients attended in two hospitals in the city of João Pessoa. Identification at species-level were performed by Matrix Assisted Laser Desorption Ionization - Time of Flight (MALDI-TOF-MS), antimicrobial susceptibility testing was performed by disk diffusion test following recommendations from the Brazilian committee on antimicrobial susceptibility testing (BrCast) and the presence of 26 beta-lactamases genes were conducted by PCR. Additionally, the clonal profile was determined by ERIC-PCR. The potential to biofilm production was evaluated by Crystal Violet (VC) staining and growth in Congo Red methods. Identification of E. coli strains generated MALDI-TOF scores ranging from 2,048 to 2,448. In the analysis of the susceptibility profile was observed resistance to at least one antimicrobial in 45.5% (5/11) of E. coli from raw milk. The high resistance rate among clinical isolates was to ciprofloxacin, 53.8% (14/26). Extended-spectrum beta-lactamase (ESBL) phenotype was present in four raw milk isolates (36.4%) and six clinical isolates (23.1%). The blaCTX-M 1/2 gene was the most prevalent in both milk samples and clinical isolates, 18.2% (2/11) and 23.1% (6/26), respectively, while blaOXA-1 was detected in one (9.1%) milk sample and four (15.4%) clinical isolates. The blaGES, blaNDM and blaSHV genes were only detected in samples of human origin, in 19.2% (5/26), 3.8% (1/26) and 3.8% (1/26), respectively. ERIC-PCR showed the presence of 31 clonal profiles, with the dissemination of clones in milk samples from different dairy farms and from different patients in the hospital environment. The results show a higher proportion of biofilm-producing strains among clinical isolates (73.1%) when compared to raw milk strains (45.4%). This is the first report of blaNDM in the state of Paraíba and shows the dissemination of CTX-M producing E. coli in animals, food, and humans. This research was based on One Health perspective on combating AMR, and the detection of these resistance determinants in different compartments in the state of Paraíba, reinforces the role of environmental factors as possible sources that can contribute to the spread of AMR. Palavras-chave: blaNDM, CTX-M, clonal profile, biomarker Agência de fomento:Instituto Nacional de Pesquisa em Resistência Antimicrobiana - INPRA (INCT – MCTI/CNPq/CAPES/FAPs nº 16/2014) | |||||