| Congresso Brasileiro de Microbiologia 2023 | Resumo: 844-1 | ||||
Resumo:Gloves are essential for the protection of professionals and patients against the risk of contamination and cross-infection in health care. Essential oils have been increasingly used in the health area due to their promising antimicrobial, antioxidant, and anti-inflammatory properties. Nonetheless, the antibacterial effect of essential oils on surface of gloves is poorly discussed in the scientific literature. Thus, the aim of this study was to evaluate the in vitro antimicrobial activity of a nano formulation with essential oils from Syzygium aromaticum (clove) and Melaleuca alternifolia (melaleuca) in latex and nitrile gloves. The antimicrobial effect was evaluated trough disc diffusion assay against the following strains: Staphylococcus aureus (ATCC 6538), Methicillin-resistant Staphylococcus aureus (ATCC 43300), Enterococcus faecalis (ATCC 29212), Streptococcus mutans (ATCC 25175), Escherichia coli (ATCC 25927), Pseudomonas aeruginosa (ATCC 27853) and Candida albicans (ATCC 90028). Fragments from latex and nitrile gloves (0.6 mm Ø) were submerged for 24 h in the nano formulation containing essential oils. Afterward, the fragments were left to dry for an additional 24 h. The disc diffusion assay was performed in triplicate, using Petri dishes (90 × 15mm) with 20 mL of Muller Hinton Agar. After the culture medium solidified, 2 mL of the standardized bacterial inoculum (108 UFC/mL) was spread over the entire agar surface using tilting and rotating movements. The excess liquid was removed with the aid of a micropipette and filter paper. After the plates were allowed to dry openly in the cabin for a few minutes, and the glove fragments were evenly spaced on the agar surface. Paper discs impregnated with 0.2 % chlorhexidine gluconate (CHX) or sterilized water (CIIPW) were used as controls in the experiment. The plates were pre-incubated at room temperature for 2 h and then incubated at 37 ºC for 24 h. The diameter of the inhibition halos were measured and expressed in millimeters (mm). The observed diameters of the inhibition halos for latex glove, nitrile glove and CHX were, respectively: S. aureus: 10 mm, 0 mm, 16.5 mm; MRSA: 13 mm, 9.7 mm, 17.5 mm; E. faecalis: 11.7 mm, 11 mm, 14.5 mm; S. mutans: 25 mm, 6.7 mm, 20.5 mm; E. coli: 9.3 mm, 0 mm, 16 mm; P. aeruginosa: 0 mm, 0 mm, 12 mm; and C. albicans: 9 mm, 0 mm, 16 mm. It should be noted that the CIIPW did not show any antimicrobial activity. In conclusion, fragments of latex glove showed antibacterial activity against all bacterial strains evaluated in this study, except P. aeruginosa. Furthermore, fragments of nitrile glove just showed antibacterial activity against MRSA, E. facecalis and S. mutans. Palavras-chave: microbiology, volatile oils, nanotechnology, fungus, bacterias Agência de fomento:Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) | |||||