| Congresso Brasileiro de Microbiologia 2023 | Resumo: 843-2 | ||||
Resumo:Cyprinodon is a genus of fish comprised of forty-nine species distributed by rivers and lakes in America, some of which are distributed in the habitats of the Dominican Republic. The Bahía de las Calderas, located in the Peravia province, constitutes one of the habitats for fish of the genus Cyprinodon, which is still being described. Despite the hypersaline conditions in the Bay, the ability of this Cyprinodon to survive has been demonstrated. The intestine of vertebrates constitutes the ideal environment for many microorganisms due to the availability of carbon, minerals, and solutes they use for their growth. In vertebrates, microbial colonization of the intestine begins after birth and is influenced by environmental factors such as the individual's habitat and diet. The microbiota of the vertebrate gut includes species that colonize the tract and a set of microbes that pass through the digestive tract transiently through food intake. PH, oxygen concentrations, and nutrient availability will influence the amount and type of bacteria present. This study uses molecular metagenomic techniques to study the intestinal microbiota of Cyprinodon from La Bahía de Las Calderas, allowing the identification of microorganisms with possible biotechnological potential and opening the way to understanding the metabolism of the species. It has been possible to confirm the ability of this Cyprinodon to survive hypersaline conditions. The composition of the microbial communities in the intestine was determined from domain to species, with bacteria representing 97% of the relative abundance. The taxonomic composition of the microbial communities identified in the intestine of Cyprinodon sp. was similar in each sample. On average, the domain with the highest presence was Bacteria (97%), followed by Viruses (2%) and Archaea (1%). The ten most significant abundances regarding edge bacteria occupy the highest proportion with 39% Proteobacteria, 25% Chlorobi, and 9% Bacteroidetes as observed in Figure 5C. In archaea, 90% belongs to Euryarchaeota, and in percentages of 5% or less, we find Crenarchaeota, Thaumarchaeota, and Korarchaeota. In this study, a large composition of Chlorobi was found, which can be studied concerning the adaptive capacity of the fish since this genus of bacteria is not highly documented in the microbiota of fish like other genera. A more significant relationship between the Cyprinodon microbiota with protein metabolism was found regarding the functional analysis. Palavras-chave: Intestinal microbiota, Cyprinodo, metagenomics, hypersalinity. Agência de fomento: Fondo Nacional de Innovación y Desarrollo Científico y Tecnológico-FONDOCYT | |||||