| Congresso Brasileiro de Microbiologia 2023 | Resumo: 836-1 | ||||
Resumo:Foodborne diseases are considered a public health problem as they can result in more than 9 million cases of illness annually. Escherichia coli has been described as one of the main foodborne pathogens in the world, being responsible for causing from mild to severe cases of gastroenteritis. These diseases significantly increase the risk of mortality, especially when it comes to multidrug resistant (MDR) bacteria, thus considerably decreasing antibiotic treatment. In this sense, alternative sources have been sought to combat microorganisms (MDR), based on the use of compounds in a secondary way. Hydralazine (HDZ) is a potent vasodilator used as an antihypertensive agent since 1952, and already present in the literature its secondary use in cancer therapy and excellent antioxidant agent. Thus, the objective of this work was exploring the antibacterial potential of HDZ against panel of food-related E. coli (C22, C27 and C55), previously isolated, and standard strain ATCC11303. The minimum inhibitory and bactericidal concentration (MIC/MBC) of HDZ was performed by the microdilution method in 96-well polystyrene plates. The growth curve assay was performed for all strains exposed to ½ and ¼ MIC values for 24 hours to verify the interference of HDZ in the bacterial growth kinetics. Assays were performed in triplicate, and results were presented as mean ± standard deviation (SD). HDZ presented MIC and MBC values of 625 μg/mL against the tested strains, except for the standard strain, which showed a value of 1,250 μg/mL. From the growth curve, it was possible to observe a significant delay in the lag phase of bacterial growth for groups treated with 1/2 and 1/4 of the MIC values, with times ranging from 0-12 h for strains C22, C27, and C55, and 0-8 h for the standard strain, in addition to a decrease in growth in the exponential phase (log). Furthermore, the analysis of the area under the curve (AUC) in the HDZ treated groups showed a significant reduction in bacterial growth against the tested strains, demonstrating the antimicrobial efficacy of hydralazine when compared to the control. The obtained results indicate that hydralazine exhibits promising antibacterial activity against pathogens isolated from food specially E.coli, making it a potential secondary tool in the field of food safety and public health, as foodborne diseases pose a significant health issue worldwide. Palavras-chave: Foodborn, Multiresistence, Bacterial, Hydralazine Agência de fomento:Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |||||