| Congresso Brasileiro de Microbiologia 2023 | Resumo: 820-1 | ||||
Resumo:A relevant number of infections caused by complex Acinetobacter calcoaceticus-baumannii (ACB) species has been observed frequently in recent years in hospitals, especially in Intensive Care Units. Such species have been showing phenotypes of resistance to carbapenems, antibiotics used as last resort in the treatment of infections by multidrug-resistant bacteria. This study aimed to characterize the isolates of Acinetobacter spp. obtained from clinical samples of patients attended at the Municipal Hospital and Health Services in Diadema, São Paulo, from May to December 2019. The identification of clinical isolates was performed by MALDI-TOF MS and the minimum inhibitory concentration (MIC) for the main antimicrobials was determined by agar dilution. Carbapenemase-encoding genes and other resistance determinants were investigated by PCR, which was also used to determine the genetic context of blaOXA-51like. The genetic variability of the isolates was determined by REP-PCR. In the study, 53 clinical isolates belonging to the ACB complex were identified, with 52 A. baumannii and one A. pittii, obtained from secretion samples (n=23), blood (n=13), urine (n=9) and catheter tip (n=8). Most of the clinical isolates (n=41, 83.68%) were obtained from patients admitted to the Municipal Hospital, causing mainly infections in the lower respiratory tract (n=14, 26.42%). It was observed 86.79% and 22.65% of isolates resistant to imipenem and colistin, respectively. Except for A. pittii, all isolates showed blaOXA-51 gene, an intrinsic oxacllinase gene. Also, blaOXA-23-like and blaOXA-24/40-like were found in 32 (60.37%) and 16 (30.18%) isolates, respectively. Both the genes were found in three isolates. There was no amplification for genes encoding metallo and/or serine β-lactamases, as well as for genes encoding extended-spectrum β-lactamases (ESBL), including those related to quinolone and colistin resistance. Twenty one isolates presented the insertion element ISAba1 located upstream of the blaOXA-51like gene. In addition, 20 clonal groups were evidenced in the clinical isolates studied. In this study, a relevant number of carbapenem-resistant A. baumannii was found causing respiratory bloodstream infections, wounds and urinary tract infections in Diadema, SP. The results obtained reinforce the need for constant surveillance and monitoring of Acinetobacter spp. Palavras-chave: Diadema, Acinetobacter, Carbapenemase, Oxacilinases Agência de fomento:Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |||||