| Congresso Brasileiro de Microbiologia 2023 | Resumo: 807-1 | ||||
Resumo:Bacteria possess a variety of protein secretion systems that are crucial for interaction with the environment, with host organisms and other members of the microbiota. Recently, much attention has been focused on the Type VI Secretion System (T6SS), which is widely distributed in Proteobacteria. There is a wide range of processes associated with them, grouped into T6SS subclasses, involved in specific functions. Some T6SSs mediate competitions by secreting toxins, which reach specific target cells, or even provide effectors that promote survival in adverse environments by acquiring metal ions. Pseudomonas aeruginosa, considered a model in T6SS studies, is a ubiquitous microorganism due to its metabolic versatility and wide adaptability to different conditions. Studies on microbial communities in extreme environments, such as oil industry systems, are essential for the control of many technical, operational and environmental issues. In this perspective, we highlight the PA1-Petro strain, isolated from oil production water in Northeast Brazil, as well as other P. aeruginosa strains of environmental origin. The objective of this work is to better understand the gene repertoire of P. aeruginosa strains, focusing on their differentials and T6SS, through the pangenome of 203 P. aeruginosa strains of environmental classification, according to the IPCD database, in 2019. The pangenome was generated with the GET_HOMOLOGUES v3.4 program, its algorithms and tools, the results were analyzed with COG, 2020 and with SecReT6 v3.0. The pangenome totalized 18,406 clusters. The most abundant COG categories in the pangenome comprises transcription, signal transduction, transport and metabolism. The largest repertoire of T6SS proteins (structural, regulatory and effector) are found in the cloud partition, where proteins are present in up to two genomes. The most present T6SS and associated proteins were ClpV, LadS, TagS, pqsA, pqsE, hcp2, VgrG2b, VgrG1b and Tle3. Given the results, we can conclude that T6SS is structurally conserved in P. aeruginosa, differing in the amount of orphan proteins in each genome, results that add to the high genomic plasticity, versatility and metabolic robustness of the species. This intraspecific diversity of the T6SS in P. aeruginosa raises important evidence about the complexity of its interactions with other bacteria and with the human host. Palavras-chave: Bioinformatics, Comparative Genomics, Pangenome, Pseudomonas aeruginosa, Type VI Secretion System Agência de fomento:CNPq | |||||