| Congresso Brasileiro de Microbiologia 2023 | Resumo: 748-2 | ||||
Resumo:Members of the Aeromonas genus are commonly found in aquatic environments and are responsible for a range of intestinal and extra-intestinal infections in both humans and animals. The adaptive capabilities of Aeromonads enable colonization of terrestrial environments and their inhabitants, thereby allowing these microorganisms to survive in various sources including soils, plants, fruits, vegetables, birds, reptiles, amphibians, and more. In humans, the genus Aeromonas is primarily associated with gastroenteritis and wound infections. We aimed to investigate the prevalence, putative virulence factors, and antimicrobial resistance of mesophilic Aeromonas strains from environmental - EN (19), animal - AN (32), and human - HU (37) origin isolated throughout 2022 at LABENT/LRNEB. We received 87 strains, of which 83 were conclusively diagnosed within this genus, distributed across 11 species (40 A. caviae, 11 A. veronii, 10 A. dhakensis, 9 A. hydrophila, 4 A. allosacharophila, 3 A. sobria, 2 A. eucrenophila, 1 A. schubertii, 1 A. media, 1 A. jandei and 1 A. bivalvium). Among the strains of human origin, we found 21 A. caviae, 4 A. dhakensis, 4 A. hydrophila, 2 A. veronii bv sobria and 1 A. schubertii. In the strains of animal origin A. caviae (7) and A. dhakensis (6) were prevalent. In the environmental strains, A. caviae (12) constituted the majority of the isolates. The presence of virulence genes in the strains suggested their potential pathogenicity: gcat (96.4%), dnase (72.3%), lip (57.8%), aerA (42.2%), act (27.7%), hlyA (25.3%), alt (21.7%), and ser (20.5%). We can highlight three strains that presented all eight virulence genes researched: 1 A. caviae from a human abscess, 1 A. dhakensis and 1 A. allosacharophila both from elasmobranchii; and also 1 A. veronii bv sobria from water with 7 virulence genes. Regarding antimicrobial susceptibility evaluation, 13 isolates showed sensitivity to all antimicrobials; 23 exhibited only intermediate resistance; 28 showed resistance to 01 antimicrobial class (R-1); 9 to 02 antimicrobial classes (R-2); 7 to 03 antimicrobial classes (R-3); 2 to 04 antimicrobial classes (R-4); and 1 showed resistance to more than 05 antimicrobial classes (R-5+). The highest resistance percentages were observed against FOX (37.3%), IPM (16.3%), and SXT (14.5%). Although at lower percentages, it is important to highlight resistance to CIP and CAZ, as well as the increasing intermediate resistance to MEM. Ten strains showed profile MDR: 4 A. hydrophila (3HU + 1AN), 3 A. caviae (2HU +1AN), 1 A. allosacharophila (AN), 1 A. sobria (AN) and 1 A.veronii bv sobria (HU). We identified 41 different virulence profiles among the tested strains; highlighting the diversity of characteristics among the strains suggests the adaptability of Aeromonas spp. to environmental conditions. Furthermore, the detection of resistant strains to cephalosporins, quinolones, and carbapenems among the isolates points to the need for research on genes that characterize antimicrobial resistance, which can be disseminated by the environment, given that the genus Aeromonas could be a reservoir of resistance determinants. Palavras-chave: Aeromonas spp.,, Virulence genes, MDR, Public Health, Zoonotic disease Agência de fomento:FIOTEC | |||||