Congresso Brasileiro de Microbiologia 2023

Congresso Brasileiro de Microbiologia 2023

Resumo: 724-1

724-1

Commiphora leptophloeos EXTRACT EFFECTS ON Staphylococcus spp. FROM MASTITIS OF RUMINANTS

Autores:

Nayara Andreo (UNIVASF - UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO, PETROLINA, PE) ; Betilde de Matos Silva (UNIVASF - UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO, PETROLINA, PE) ; Priscila Izidro de Figueirêdo (UFRPE - UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO, RECIFE – PE ) ; Raíssa Alves Santos (UNIVASF - UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO, PETROLINA, PE) ; Edinaene Alves Santos (UNIVASF - UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO, PETROLINA, PE) ; Marcos Felipe dos Santos Ferreira (UNIVASF - UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO, PETROLINA, PE) ; Danillo Sales Rosa (UFRPE - UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO, RECIFE – PE ) ; Mateus Matiuzzi da Costa (UNIVASF - UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO, PETROLINA, PE)

Resumo:

Staphylococcus spp. are the main pathogenic agents involved in mastitis, a disease that affects goats and dairy cattle, causing economic and public health losses. These bacteria can form biofilm, one of the antimicrobial resistance mechanisms, and it is important to develop alternative methods capable of combating this structure. In this sense, research with plants bioactive products is highlighted, as Commiphora leptophloeos, a native species of Caatinga, whose extracts have an important antimicrobial action. Thus, the aim was to evaluate the effect of Commiphora leptophloeos extract on formed biofilms by Staphylococcus spp. from mastitis of goats and dairy cattle. The study was carried out at the Laboratory of Animal Microbiology and Immunology at Federal University of Vale do São Francisco, Petrolina - PE. Ten S. aureus (six bovine and four caprine) and eight S. warneri isolates were used, in addition to two and one strains, respectively, from the American Type Culture Collection. Bacteria biofilm production was quantified by the adherence to polyethylene microplates method, and staining with gentian violet. The effect of the C. leptophloeos extract on the biofilm formation process and on consolidated biofilm was evaluated using concentrations of 1/2 and 1/4 of minimum inhibitory concentration. Regarding quantification and biofilm formation interference, the isolates were classified as: non, weak, moderate and strong producer. As for the consolidated biofilm, it was evaluated if there was an increase, decrease or absence of difference in biofilm after 24 h of extract exposure. The tests were performed in technical and biological triplicate. S. aureus dairy cattle origin were moderate to strong biofilm producers, while S. aureus and S. warneri of goat origin ranged from weak to moderate biofilm producers. In the two tested concentrations, C. leptophloeos was able to modify the isolates classification from strong and moderate biofilm producers to a weak producer. However, the extract was not able to reduce consolidated biofilm. Therefore, is evident the potential action of C. leptophloeos extract in reducing biofilm formation by Staphylococcus spp., proving to be an alternative to antimicrobials use.

Palavras-chave:

Bacterial, Imburana, Mammary infection , Virulence

Agência de fomento:

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE).