| Congresso Brasileiro de Microbiologia 2023 | Resumo: 713-1 | ||||
Resumo:Mastitis is an infection primarily caused by bacteria in the mammary gland (IMI) of goats and other mammals. In goats, the most common agents associated with IMI are Coagulase-Negative Staphylococci, including Staphylococcus warneri. While studies in cattle have demonstrated alterations in the chemical components of milk, research on goats is limited. The objective of this study was to investigate the effects of S. warneri-induced IMI on goat milk and its microbiota. To assess the concentration of chemical elements, we performed energy-dispersive spectroscopy using scanning electron microscopy (SEM), and bacterial identification was conducted using the chromogenic agar Accumast® (AC). Eight primiparous Alpine breed goats were used. Infection with S. warneri was induced in six animals (C, D, E, F, G and H) in the right teat (RT) of the udder. Animal (B - Control) received saline inoculation in the RT, while animal (A - Control) underwent no intervention. The milk samples were collected at two different periods during the infection induction phase: (Period - P0) - two days before inoculation; (Period - P8) - the eleventh day of infection. Clinical evaluations and standard tests were performed for the diagnosis of IMI. Subsequently, bacterial culture was performed on AC, which has three independent selective media for bacterial identification. Additionally, the intensities (counts per second) of ten chemical elements (Sodium - Na, Magnesium - Mg, Phosphorus - P, Chlorine - Cl, Potassium - K, Calcium - Ca, Iron - Fe, Copper - Cu, Zinc - Zn and Molybdenum - Mo) were analyzed by SEM. The statistical significance of the data was evaluated using the R program. Ca and Fe showed significant differences in the analyzed periods (P0 and P8) for animal (A), while Na, Mg, P, Cl, K, and Ca were significant for animal (B), as well as for the inoculated animals. Cu, Zn and Mo demonstrated differences only between the periods for animals inoculated with S. warneri (Fig 1). No changes in identified bacterial genera were observed in goats (A) and (B) using AC (Table 1). However, five out of six goats inoculated with S. warneri demonstrated microbiota dysbiosis, with an increase in isolated bacterial genera at P8, such as Enterobacter sp., Pseudomonas sp., Enterobacter sp., and Klebsiella sp. Therefore, these results indicate that a simple intervention, such as the use of saline solution (goat - B), may already be sufficient to generate significant alterations in milk elements, possibly due to stress, immune response, or hormonal response. Furthermore, the number of identified bacterial isolates may be related to the number of significant element counts, as observed in the immune response and changes caused by the mastitis condition. Palavras-chave: disease, ruminant, microbiota Agência de fomento:CNPq – Conselho Nacional de Desenvolvimento Científico e Tecnológico, Fapemig - Fundação de Amparo à Pesquisa do Estado de Minas Gerais e Capes - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior. | |||||