| Congresso Brasileiro de Microbiologia 2023 | Resumo: 673-1 | ||||
Resumo:Cystic Fibrosis (CF) patients suffer from recurrent pulmonary bacterial infections and microbiological communities in the airway may be associated with clinical conditions and exacerbation. Some pathogens (as Pseudomonas aeruginosa, Staphylococcus aureus and Burkholderia cepacia complex) are commonly associated with pulmonary colonization in CF patients. The predominance of a specific pathogen and the decrease in lung function mean that many patients require lung transplantation. The main of this study is described a microbiome lung profile from a bilateral lung transplanted patient. A young-aged man with Cystic Fibrosis, heterozygous for others non-delta F mutations in used of support of oxygen therapy and chronically colonized by Pseudomonas aeruginosa was admitted for a bilateral lung transplantation on February of 2020. The patient developed a pneumoniae by Acinetobacter baumannii/calcoaceticus complex during hospitalization. After surgical procedure, three sputa specimens were collected: at February 28th, March 6th and March 13th; and submitted to a microbiome sequencing. Library of 16S rRNA was prepared according to a standard protocol using a V3V4 region and sequenced in Illumina MiSeq (Illumina, San Diego, US) and the amplicon sequence variants (ASV) were obtained. According to microbiome analysis, two of three specimens presented a high abundance of the genus Acinetobacter, even more the first sputum collected is composed almost exclusively by this genus. The third specimen has the higher diversity of genus compared with others: Acinetobacter, Rothia, Entereoccus, Staphylococcus and Pseudomonas.The emergence of culture-independent techniques, such as sequencing of the 16S rRNA gene, has demonstrated that low respiratory tract may be composed of a diverse microbial community and this leads to new hypotheses related to the pathogenesis of the disease. Noteworthy, an eventual decrease in diversity of the microbiome is usually associated with an increase in the dominance of a specific taxon, as observed in two sputa analyzed. Despite the genus Acinetobacter is not a pathogen commonly associated with the pulmonary infection in patients with CF, the high prevalence indicates that this bacterium was probably associated to a decrease of lung function. Moreover, the different profile of each specimen, demonstrate that microbiome is transitory and unique for each patient. Palavras-chave: Cystic Fibrosis, Microbiome, Acinetobacter baumannii, bilateral lung transplantation Agência de fomento:INPRA - Instituto Nacional de Pesquisa em Resistência Antimicrobiana - Brazil (CNPq/ INCT); Fundo de Incentivo à Pesquisa e Eventos do Hospital de Clínicas de Porto Alegre (FIPE/HCPA). | |||||