| Congresso Brasileiro de Microbiologia 2023 | Resumo: 672-1 | ||||
Resumo:Tupana carbonate ridge and Alpha Crucis carbonate ridge are two recent described regions of carbonate ridge located in the Santos Basin, Brazil, that were recently discovered. Therefore, little is known about the microbial communities that play ecological roles in these regions. To study the microbial communities associated with these carbonate regions, we collected deep sea sediment with box-corer and inoculated in culture medium NMS + 3.5% NaCl and methane, at 20 ºC, for 8 months. In cultures where the growth of methanotrophic microorganisms was confirmed by sequencing the 16S rRNA gene, metagenomic sequencing was performed using the Illumina Hiseq platform. The data were analyzed through bioinformatics analysis, to recover MAGs we performed assembly (MetaSpades) and binning (MaxBin2, MetaBAT2 and CONCOCT). For taxonomic analysis we used GTDB-tk v 2.0 and for functional annotation we used RAST v1.073, KEGG and DRAM. We obtained a total of 90 MAGs, these being 78 high quality MAGs, 8 medium quality MAGs and 4 low quality MAGs. The taxonomic annotation revealed the presence of 18 families of the Domain Bacteria, distributed in 3 different Phyla (Proteobacteria, Bacteroidetes and Actinobacteria). 74 MAGs were taxonomically assigned as belonging to the Phylum Proteobacteria, followed by 14 from the Phylum Bacteroidota and one from the Phylum Actinobacteriota, distributed in the classes Gammaproteobacteria (46 MAGs), Alphaproteobacteria (27 MAGs), Bacteroidia (13 MAGs) and Actinobacteria (1 MAG). Functional annotation revealed that MAGs identified in the Alpha Crucis carbonate mound region have genes associated with methanotrophy. Both the MAGs found in the Alpha Crucis carbonate ridge region and in the Tupana Carbonate ridge presented genes associated with the conversion of CO2 into methane and the conversion of acetate into CH4. The MAGs associated with these processes belong to the genera Methylophaga, Shewanella,Cobetia,Halomonas and Alcanivorax. The conversion of trimethylamine to dimethylamine was also an identified process and is associated with the genera Paracoccus, Nitratireductor and Pseudoceanicola. Regarding nitrogen metabolism, Alcanivorax, Pseudomonas, Sneathiella,Pseudoalteromonas, Rhizobiales and Marinobacter showed genes related to the conversion of nitrate into nitrite. Genes associated with the conversion of nitric oxide into nitrous oxide were also detected in some genera such as Alcanivorax, Methylophaga, Marinobacter, Pseudomonas and Alteromonas. These analyzes reveal the first results generated on the functional capacity of MAGs associated with carbonate regions in the Santos Basin and reinforce the importance of studies to understand the role of microorganisms in biogeochemical cycles in the deep sea. Palavras-chave: Metanotrophy, MAGs, Santos Basin, Functional metabolism, Carbonate mounds Agência de fomento:Shell Brasil - project registered as ANP 21012-0, “MARINE LIFE - BMC - OIL, AND GAS SEEPS (BIOIL) | |||||