| Congresso Brasileiro de Microbiologia 2023 | Resumo: 668-1 | ||||
Resumo:Soils represent one of the greatest biological diversity reservoirs on earth. The microbial communities, composed of a range of species and genera, play fundamental roles in maintaining and improving soil quality. The association between bacteria occurs in many different ways, some coexist beneficially, while others can inhibit the development of other bacteria. This work aimed to evaluate the compatibility of Bacillus megaterium (B119), B. subtilis (B2084), and B. thuringiensis (B116) strains with Azospirillum and Bradyrhizobium strains commonly used in Brazilian commercial inoculants. The experiment was performed in the Microbiology and Soil Biochemistry laboratory, established in Embrapa Milho e Sorgo. Strains of Bacillus and Azospirillum genera belong to Embrapa Milho e Sorgo's Multifunctional Microorganisms Collection, and the Bradyrhizobium genus belongs to Embrapa Soja's Multifunctional Microorganisms Collection. The compatibility tests were conducted through the Cross-Streak method considering two different culture mediums: YMA (Yeast, Mannitol, Agar) and PDA (Potato-Dextrose-Agar). For YMA, combinations were tested between Bacillus strains with B. diazoefficients (Semia 5080), B. elkani (Semia 5019), B. elkani (Semia 587), B. japonicum (Semia 5079). Considering the PDA medium, the combinations between Bacillus and Azospirilum sp. 2083 (V5), and Azospirilum sp. 2084 (V6) were tested. The Petri dishes were incubated in a bacteriological oven, in triplicates, at 28º C, and the evaluation was performed after six days of incubation. The Pseudomonas aeruginosa x Paenibacillus ottowiiv interaction was determined as a control treatment, once has shown incompatibility in previous tests, to illustrate the inhibition through this method, which can be evidenced by the formation of inhibition zones in the strains-interaction areas, as represented by the figure below. Obtained results are qualitative, based on the visual analysis between strains' contact zones. No sign of inhibition zones was observed during the test, suggesting all tested bacteria can simultaneously grow, showing no antagonistic effects. The compatibility between Bacillus B116, B119, and B2084 strains with Azospirilum and Bradyrhizobium, allows the development of efficient strategies to improve inoculants' formulation to its integrated use in the farmer's management practices, expanding its mechanism of action to guarantee suitable productivity through soil's microbial diversity. Palavras-chave: antagonism, bioprospection, Cross-streak Agência de fomento:Embrapa Milho e Sorgo | |||||