| Congresso Brasileiro de Microbiologia 2023 | Resumo: 636-1 | ||||
Resumo:Bacterial antibiotic resistance is a current public health problem affecting humans and animals. The objective of the study was to identify Gram-negative bacilli (GNB) resistant to broad-spectrum cephalosporins present in freshly emitted feces of healthy wild and exotic birds from a zoo in Brazil, and healthy wild birds of free-living received by the zoo. Fifty-two from the zoo and 28 free-living birds from sixteen different orders were investigated. Feces were selected on MacConkey agar with 2μg/mL cefotaxime. MALDI-TOF MS and Klebsiella MALDI TypeR (https://maldityper.pasteur.fr/) were used for bacterial identification. The antimicrobial susceptibility was tested by disk diffusion and a double-disk synergy test was performed for Enterobacterales isolates. The genomic similarity and search for plasmids in DNA were carried out by XbaI-PFGE and S1-PFGE, respectively. PCR was performed to search for beta-lactamase genes. Of the 80 birds included in the study, 20 from the zoo (38%) and 5 free-living birds (18%) did not present cefotaxime-resistant GNB. Among the 32 remaining birds from the zoo, E. coli was the most isolated species; among the 23 free-living birds, Klebsiella spp. was the most isolated. All Enterobacterales from the zoo (n=34) and free-living (n=31) showed the phenotypic profile of ESBL. The majority of the zoo and free-living birds were resistant to enrofloxacin. blaCTX-M-group-1, blaTEM-like and blaSHV-like genes were the most detected, while blaKPC-like was detected in K. pneumoniae complex from a free-living bird. According to genomic similarity results, some similar profiles were found between E. coli isolates from the zoo and free-living birds. Identical profiles were found in interspecies E. coli, K. pneumoniae complex, and E. cloacae isolates in birds without known contact with the zoo or free-living birds. S1-PFGE analysis revealed that several isolates had one to three plasmids DNA with sizes from 15 to 350 kb. These results reinforce the hypothesis that the cefotaxime-resistant GNB among the studied birds might be related to human-animal vectors or the acquisition of environmental clones. Palavras-chave: Antimicrobial resistance, Beta-lactamases, One Health Agência de fomento:National Council for Scientific and Technological Development (CNPq) | |||||