Congresso Brasileiro de Microbiologia 2023

Congresso Brasileiro de Microbiologia 2023

Resumo: 597-1

597-1

GENOMIC ANALYSIS OF Azospirillum STRAINS FOR IDENTIFYING GENES RELATED TO PLANT GROWTH PROMOTION

Autores:

Vitoria Palhares Ribeiro (UFSJ - UNIVERSIDADE FEDERAL DE SÃO JOÃO DEL REI) ; Lourenço Vitor Silva Ferreira (UFSJ - UNIVERSIDADE FEDERAL DE SÃO JOÃO DEL REI) ; Samuel Felipe Azevedo Galvão (UFSJ - UNIVERSIDADE FEDERAL DE SÃO JOÃO DEL REI) ; Sylvia Morais de Sousa (CNPMS - Empresa Brasileira de Pesquisa Agropecuária Milho e Sorgo) ; Eliane Aparecida Gomes (CNPMS - Empresa Brasileira de Pesquisa Agropecuária Milho e Sorgo) ; Ubiraci Gomes de Paula Lana (CNPMS - Empresa Brasileira de Pesquisa Agropecuária Milho e Sorgo) ; Christiane Abreu Oliveira Paiva (CNPMS - Empresa Brasileira de Pesquisa Agropecuária Milho e Sorgo) ; Ivanildo Evòdio Marriel (CNPMS - Empresa Brasileira de Pesquisa Agropecuária Milho e Sorgo)

Resumo:

Plant growth-promoting bacteria (PGPB) is an eco-friendly strategy to increase crop yield by improving nutrient availability in sustainable agricultural systems. Among the PGPB, the genus Azospirillum has stood out, comprising a group of diazotrophic, Gram-negative, microaerophilic, and facultative endophytic bacteria widely distributed in the environment. The objective of this work was to characterize the genome of two Azospirillum strains of the Collection of Multifunctional Microorganisms of Embrapa Maize and Sorghum, isolated from the sorghum stem (CMS1626) and maize rhizosphere (CMS2142). Both strains, capable of fixing nitrogen, were sequenced using the Illumina HiSeq 4000 platform and 150-paired end strategy by Beijing Genomics Institute (BGI). Sequenced reads were assembled with SPAdes and verified by BUSCO software. The genomes were annotated using PROKKA (version 1.8) and RAST (version 2.0) software. The phylogenetic analysis was performed with the entire sequenced genome using TYGS software. The total size of the genomes was 7.75 x 106 and 6.98 x 106 base pairs (bp), which assembled 67 and 68 contigs for CMS2142 and CMS1626, respectively. The GC content was 68.43% and 68.59%, with an N50 2.71 x 105 and 3.39 x 105 bp, and the number of coding sequences per annotated genome was 6.5 x 103 and 7.3 x 103 for CMS2142 and CMS1626, respectively. The genome-based analysis showed that strain CMS1626 belongs to the Azospirillum argentinense group and strain CMS2142 to the A. brasilense group. The genome comparison between strains revealed many differences in the number of genes related to plant growth promotion, such as phosphorus, nitrogen metabolism, and organic acid production. The strains also differed for other functional categories, including motility and chemotaxis. These Azospirillum strains have potential as microbial inoculants to increase crop production and enhance plant adaptation to different environmental conditions.

Palavras-chave:

Plant growth-promoting bacteria (PGPB), genome, sequencing

Agência de fomento:

Embrapa Milho e Sorgo