| Congresso Brasileiro de Microbiologia 2023 | Resumo: 575-1 | ||||
Resumo:Oysters are fresh foods that often are consumed in natura, in which the presence of clinically relevant microorganisms that are multidrug-resistant represents a public health problem that directly impacts the quality of these products. Samples of 15 adult oysters were collected from oyster farmers located in the north bay (n=3) and south bay (n=3) of Florianópolis/SC, as well as from the establishments (restaurants and fish markets) where these producers supply. The oysters from each location were homogenized and then incubated to Tryptic Soy enrichment broth (TSB), and then incubated at 37°C under agitation for 6 hours. From each enrichment, aliquots were then transferred to a new TSB medium supplemented with cefotaxime (2 µg/mL), ertapenem (0.5 µg/mL), gentamicin (2 µg/mL) and ciprofloxacin (0.5 µg/mL) separately, and incubated at 37°C overnight. After that, samples were seeded in MacConkey agar, containing an antimicrobial disk equal to that used in the selective pressure (cefotaxime 30 µg; ertapenem 10 µg; gentamicin 10 µg and ciprofloxacin 5 µg) and incubated at 37°C for 24h. Each morphologically distinct colony, which grew close to the antimicrobial disk, was isolated and identified by MALDI-TOF. Subsequently, an antimicrobial susceptibility test was performed using the disk diffusion method, as recommended by BrCAST for Enterobacterales. For detection of Extended Spectrum Beta-Lactamases (ESBL) phenotype, the combined disk method was used, and the isolate was considered multidrug-resistant when it showed resistance to at least three different classes of tested antimicrobials (β-lactams, aminoglycosides, quinolones, tetracyclines). In total, 64 Enterobacterales with antimicrobial resistance profiles were isolated, 35 isolated (55%) in oysters collected directly from the sea and 29 isolated (45%) from commercially available oysters. Although the highest number of antimicrobial-resistant Enterobacterales were isolated from samples without direct human contact, the proportion of isolates with ESBL and multidrug-resistant phenotypes was higher in commercial oysters, with 79% of commercial oysters having the ESBL phenotype and 72% being multiresistant, compared to 54% and 57% of sea oysters, respectively. The production location of the oyster seems to have a greater impact on the presence of antimicrobial-resistant Enterobacterales. At the three oyster collection locations in the northern bay, only 4 antimicrobial-resistant Enterobacterales were isolated, none of which had an ESBL or multi-resistant phenotype. Similarly, the commercial establishments supplied by these producers showed low counts with only 3 Enterobacterales being isolated, two of which were ESBL and multidrug-resistant. In contrast, oysters collected from the southern bay yielded 31 Enterobacterales isolates, of which 19 were ESBL and 20 were multidrug-resistant, while in the stores supplied by these producers, 26 Enterobacterales were isolated, of which 21 were ESBL and 19 were multidrug-resistant. Thus, the results demonstrate that the number of antimicrobial-resistant isolates is more influenced by the water quality during the production process, than by the storage process or human handling process itself. However, the greater proportion of isolates with ESBL and multidrug-resistant phenotype in commercially sold oysters indicates that the manipulation process of this product affects the presence of antimicrobial-resistant Enterobacterales. Palavras-chave: Antimicrobial Resistance, Fish Markets, Enterobacterales, One health, Oyster Agência de fomento:Fundação de Amparo à Pesquisa e Inovação do Estado de Santa Catarina (FAPESC); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |||||