| Congresso Brasileiro de Microbiologia 2023 | Resumo: 494-1 | ||||
Resumo:Due to the growth of antimicrobial resistance in recent years, the World Health Organization (WHO) has provided a list of 12 antibiotic-resistant pathogens, divided into three categories, that are priority for the production and development of new antimicrobials. Among these, carbapenemase, even as Extended-spectrum β-lactamase-producing Enterobacteriaceae, are of critical priority. Furthermore, WHO created the “Tricycle Project”, a global surveillance plan for ESBL-producing Escherichia coli (ESBL-Ec), where One Health approach aims to standardize methodologies for detection and monitoring ESBL-Ec prevalence in humans, animals and the environment. E. coli is a commensal bacterium that inhabits the lower intestinal tracts of humans and animals, and can cause intestinal infections, according to the pathotype present in the host, and can reach the environment through feces or untreated sewage. Whereas antibiotic resistance is a growing threat and considering that the environment is a reservoir for a diversity of antibiotic resistance genes, the aim of this study was to detect potentially ESBL-Ec in marine environments to evaluate the E. coli resistome in three different ecosystems. Water samples were collected and analyzed monthly from Icaraí, Piratininga and Jurujuba beaches (Niterói, RJ) in the period from July 2022 to October 2022, and were inoculated on MacConkey, EMB and CLED agar. After identification through biochemical tests and/or Mass Spectrometry by using MALDI-TOF, screening was performed for ESBL-Ec and KPC (Klebsiella pneumoniae Carbapenemase) producing E. coli in ESBL and KPC Chromogenic Agar. All 40 strains tested were positive for the potential production of ESBL (three from Jurujuba beach, nine from Piratininga beach, and 28 from Icaraí beach), and five were positive for the potential production of KPC (one from Icaraí beach, one from Jurujuba beach, and 3 from Piratininga beach). All E. coli isolates were submitted to antimicrobial susceptibility testing by disc diffusion method (according to the Brazilian Committee on Antimicrobial Susceptibility - BrCAST) for 18 different antimicrobials, between β-lactams, aminoglycosides, fluoroquinolones, nitrofurans and sulfonamide; 20.0% of the strains showed resistance to gentamicin; 17.5% were resistance to cefepime and ampicillin and 12.5% to ertapenem, cefazolin and ceftazidime. All strains were susceptible to levofloxacin and one strain was multidrug resistant, showing resistance to cefazolin, ceftazidime, ciprofloxacin and gentamicin. Double-disk synergy test (DDST) was performed with three strains that showed an inhibition zone under 21 mm for cefotaxime, but all of them were negative. Twelve β-lactams resistant strains were investigated for blaCTX-M-2, blaCTX-M-8 and blaKPC genes by PCR and no one was positive for these β-lactamase enzymes. Although no ESBL-EC were confirmed in this period of analyzes, the detection of MDR E. coli indicates that there is the maintenance of its resistome, even though the quality of water sources were acceptable for recreational activities. The presence of MDR bacteria in the environment highlights the importance of maintaining public health, since horizontal gene transfer between species can occur in the environment. Palavras-chave: Antimicrobial resistance, ESBL, Escherichia coli, Multidrug resistance, One Health Agência de fomento:Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) | |||||