| Congresso Brasileiro de Microbiologia 2023 | Resumo: 485-1 | ||||
Resumo:Companion animals, such as dogs and cats, can be colonized in their intestinal microbiota, by beta-lactam-resistant bacteria. These bacteria can be acquired through human contact and/or other animals, food, or through the environment. Studies on beta-lactam-resistant Gram-negative bacilli present in the intestinal microbiota of dogs and cats are relevant to enhance knowledge about the zoonotic potential of these bacteria that can affect human and animal health and the environment. The study aimed to investigate the presence of Gram-negative bacilli resistant to beta-lactams in dogs and cats without clinical manifestations and to determine the antimicrobial resistance profile. Fifty-eight rectal swabs from 20 dogs and 38 cats, without signs of diarrhea and that had not received antimicrobials for 4 weeks before the study, were collected. The sample was selected on MacConkey agar with 2μg/mL cefotaxime. MALDI-TOF MS and Klebsiella MALDI TypeR (https://maldityper.pasteur.fr/) were used for bacterial identification. The antimicrobial susceptibility was tested by disk diffusion; a double-disk synergy test (DDST) was performed for Enterobacterales. Twenty out of 58 samples showed cefotaxime-resistant BGN, 14 were from dogs and 6 were from cats. The dogs presented 20 bacterial isolates and the cats 9 isolates, counting for 29 bacteria isolates. Twenty-two bacteria were identified as 9 Escherichia coli, 1 Klebsiella pneumoniae, 1 Pseudescherichia vulneris, 4 Pseudomonas aeruginosa, 1 Pseudomonas putida, 1 Pseudomonas monteilii, 1 Pseudomonas stutzeri, 1 Acinetobacter vivianii, 1 Achromobacter mucicolens, 1 Stenotrophomonas maltophilia, 1 Ochrobactrum anthropi. E. coli was the most isolated bacteria, followed by Pseudomonas spp. The majority of Enterobacterales isolates showed ESBL production by DDST and were resistant to cefotaxime, cefepime, amoxicillin/clavulanate, aztreonam, ciprofloxacin, levofloxacin, tetracycline. All the isolates were susceptible to ertapenem. Although Pseudomonas aeruginosa is intrinsically resistant to cefotaxime, it was included in the study as well as other Pseudomonas spp. because it is Gram-negative bacilli and the investigation of resistance to other antimicrobials of clinical-veterinary importance is necessary. All seven Pseudomonas spp isolates were resistant to aztreonam, ciprofloxacin, levofloxacin, and cefoxitin. Dogs and cats have been described as potential reservoirs of antimicrobial resistance bacteria and due to the close human contact, the prevalence of these bacteria in the obtained samples is worrying from an epidemiological point of view, since transmission between humans and animals is likely to occur. Palavras-chave: Antimicrobial resistance, Dogs, Cats, One Health Agęncia de fomento:National Council for Scientific and Technological Development (CNPq) | |||||