| Congresso Brasileiro de Microbiologia 2023 | Resumo: 437-1 | ||||
Resumo:ABSTRACT
Cervical cancer is the fourth most common neoplasm and one of the leading causes of death among women. Human papillomavirus (HPV) infection is known to be the most important risk factor. However, studies indicate that there are other related risk factors, including the presence of the Epstein-Barr Virus (EBV) simultaneously with high-risk HPV in the uterine cervix. During latent infection, EBV can express a set of genes, including LMP1, whose product is considered its main oncoprotein. Furthermore, strains that have a 30bp deletion in the LMP1 gene appear to be more virulent. The present study aimed to analyze the prevalence of EBV and HPV16 in samples of cervical carcinoma, and the frequency of the 30bp deletion of the LMP1 gene. The specimens were collected, during hysterectomies, in two oncological hospitals in the State of Ceará, and kept in a freezer at -80°C until the DNA extraction. EBV was detected by polymerase chain reaction (PCR) using primers that flank the region of occurrence of aforementioned deletion. The amplification products appeared as bands of 131bp or 161bp. Nested-PCR was also performed for HPV detection and genotype 16 was later confirmed with specific primers. Out of 97 cases analyzed, 27 (27.83%) were positive for EBV, of which 15 cases showed a band of 131bp and 12 cases of 161bp. Furthermore, 04 cases exhibited both bands, indicating the presence of more than one viral strain. All 97 cases were HPV-positive by Nested-PCR, of which 39 (40.2%) carried HPV16. Of the 27 EBV-positive cases, 13 were co-infected with HPV16 (p=0.488). The PCR-EBV and 30bp deletion results were confronted with data on differentiation grade and tumor staging of the samples, however, without statistical significance. Of the 27 EBV-positive cases, 16 (59.2%) were moderately and 6 (22.2%) were poorly differentiated. Most cases were of tumor stage I and II. Our findings show that EBV is present in about 1/4 of the patients studied and that most of the strains found had a 30bp deletion, however, without demonstrating a more aggressive tumor profile. It is worth noting the occurrence of co-infection with two strains of EBV. More studies are still needed to understand the real role of EBV and its relationship with HPV in the genesis and progression of cervical carcinomas, highlighting case-control and survival studies.
Key-words:Cervical Carcinoma, EBV, PCR, deletion, oncoviruses
Development Agency: Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico - FUNCAP; Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq.
Figure X - 2% Agarose Gel Electrophoresis with PCR Product for EBV Detection:
https://ibb.co/JRLf2h1.
Palavras-chave: Cervical Carcinoma, EBV, PCR, Deletion , Oncoviruses Agência de fomento:Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico - FUNCAP; Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq | |||||