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Congresso Brasileiro de Microbiologia 2023
 		Resumo: 391-2

391-2

DEFENSIN-LIKE PEPTIDES FROM CHILLI PEPPER (Capsicum chinense) CAUSE INHIBITION OF THE GROWTH AND MORPHOLOGICAL ALTERATIONS IN THE CELLS OF Colletotrichum scovillei FUNGI

Autores:
Larissa Maximiano Resende (UENF - Universidade Estadual do Norte Fluminense Darcy Ribeiro) ; Arielle Pinheiro Bessiatti Fava Oliveira (UENF - Universidade Estadual do Norte Fluminense Darcy Ribeiro) ; Érica de Oliveira Mello (UENF - Universidade Estadual do Norte Fluminense Darcy Ribeiro) ; Gabriel Bonan Taveira (UENF - Universidade Estadual do Norte Fluminense Darcy Ribeiro) ; Thaynă Amanda Melo Souza (UENF - Universidade Estadual do Norte Fluminense Darcy Ribeiro) ; Felipe Figueiroa Moreira (UENF - Universidade Estadual do Norte Fluminense Darcy Ribeiro) ; Sérgio Henrique Seabra (UENF - Universidade Estadual do Norte Fluminense Darcy Ribeiro) ; André Teixeira Ferreira (UENF - Universidade Estadual do Norte Fluminense Darcy Ribeiro) ; Jonas Enrique Perales Aguilar (UENF - Universidade Estadual do Norte Fluminense Darcy Ribeiro) ; Andre de Oliveira Carvalho (UENF - Universidade Estadual do Norte Fluminense Darcy Ribeiro) ; Rosana Rodrigues (UENF - Universidade Estadual do Norte Fluminense Darcy Ribeiro) ; Valdirene Moreira Gomes (UENF - Universidade Estadual do Norte Fluminense Darcy Ribeiro)

Resumo:
Fungi are one of the most common and important plant pathogens. Natural antifungal component research appears to be an option for mitigating this problem, yielding knowledge that can be exploited to generate resistant cultivars or natural control agents. The peppers and chilies are a promising source of antimicrobial peptides (AMPs). We found two peptides from the defensin family in an antifungal fraction extracted from Capsicum chinense pepper fruits in this investigation. Furthermore, we discovered the mechanism through which these AMPs inhibited the growth of Colletotrichum scovillei. The amp-rich extract was extracted from the pericarp of C. chinense peppers and subjected to ion exchange, molecular exclusion, and reverse phase. The peptide fractions obtained were tested for their ability to suppress the growth of C. scovillei. We investigated the endogenous increase in reactive oxygen species (ROS), the loss of mitochondrial functioning, and the ultrastructure of hyphae. In the final stage of the investigation, mass spectrometry was used to identify the peptides of the fraction that exhibited the potential to suppress fungal development. The peptides isolated from the G3-fraction using molecular exclusion chromatography were separated using reverse phase chromatography. F1, F2, F3, F4, and F5 fractions were obtained. The F1 fraction suppressed C. scovillei growth by 90, 70.4, and 44% at 100, 50, and 25 µg/mL, respectively. At 24 hours, the IC50 and minimum inhibitory concentration were determined to be 21.5 µg/mL and 200 µg/mL, respectively. We found an increase in ROS, which may have resulted in an oxidative burst, loss of mitochondrial functioning, and cytoplasm retraction, as well as an increase in autophagic vacuoles. MS/MS analysis of the f1-Fraction indicated that it was rich in two defensin-like proteins. F1-Fraction was also found to inhibit the activity of insect &alfa;-amylases. In summary, the F1 fraction of C.chinense exhibits antifungal activity against a major pepper pathogen that causes Anthracnose. These defensin-like compounds are promising prospects for further research into antifungal and insecticide biotechnology applications. Contributing to the development of technology to improve pepper farming based on research into proteins involved in the plant's defensive process.

Palavras-chave:
 AMP, Anthracnose, Defensin, Fungi


Agęncia de fomento:
CAPES