| Congresso Brasileiro de Microbiologia 2023 | Resumo: 350-2 | ||||
Resumo:Antimicrobial resistance (AMR) is a way in which microorganisms manage to evade drugs that are intended to eradicate them. In fact, AMR is a global health problem which brings increases hospital costs as well as deaths related to it. In addition, microorganisms can form biofilms to survive to the antimicrobial therapies. Biofilms are defined as microbial community insert in a self-produced extracellular polymeric matrix attached to a biotic or abiotic surface. Staphylococcus aureus and Staphylococcus epidermidis are important pathogens which are related to healthcare-associated infections and commonly exhibit biofilm formation as a form of AMR. Therefore, it is necessary to search for new antimicrobials that can fight infections caused by resistant bacteria, as well as the biofilm produced by them. Medicinal plants, such as Copaifera spp, have several biological properties previously reported, included antimicrobial activity against resistant species. The aim of this study was to test the activity of the crude oil, non-volatile fraction and the main compound (Hardwick acid) of the specie C. pubiflora Benth. against S. aureus ATCC 25923, S. aureus ATCC 700698 (Methicillin resistant S. aureus) and S. aureus JKD 6008 (Vancomycin-intermediate S. aureus), S. epidermidis ATCC 12228 and S. epidermidis 35984 (Methicillin resistant S. epidermidis). Antimicrobial activity was tested by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) determination in microdilution assays. Regarding antibiofilm activity, the methodology used was the enumeration of colony forming units (CFU), though the counting of viable cells. In summary, the crude oil showed MIC and MBC values ranged from 31,25 to 1000 μg/mL and Hardwick acid the values ranged from 125 to 500 μg/mL for all strains tested. In the enumeration of viable cells from biofilms, the crude oil caused a reduction of CFUs, compared to the control, that ranged from 0,99 to 4,38 logs, and for the Hardwick acid was observed a reduction of CFUs ranged from 0,69 to 4,25 logs. It has been demonstrated that crude oil, as well as Hardwick acid, showed significative antibacterial and antibiofilm activity against the Gram-positive species tested. Thereby, the compound extracted of C. pubiflora Benth. can be a natural alternative against infections caused by S. aureus and S. epidermidis. Palavras-chave: Staphylococcus aureus , Copaifera pubiflora , Biofilms, HAI, Natural Plants Agência de fomento:CAPES, CNPq, FUNCAP. | |||||