| Congresso Brasileiro de Microbiologia 2023 | Resumo: 350-1 | ||||
Resumo:Healthcare-associated Infections (HAIs) affect patients under medical care which causes several deaths and costs concerning to hospitalization. Antimicrobial resistance (AMR) makes the treatment of HAIs more difficult, as antimicrobial therapies become ineffective. Furthermore, microorganisms can develop structured communities inserted in a self-produced extracellular polymeric matrix that have the property of adhering to biotic and abiotic surfaces, called as biofilms. Staphylococcus aureus is an important pathogen of clinical interest because it is recurrently associated to several HAIs and commonly presents AMR. In addition, a lot of infections are related to biofilms caused by S. aureus. Thus, there is a clear need to find new compounds that can fight infections caused by resistant microorganisms, as well prevent or control their biofilm formation. In this sense, the use of natural plants can be an alternative, since many have proven biological activities in the literature, as is the case of Copaifera spp. The present study aimed to evaluate the antibacterial and antibiofilm activity of the non-volatile fraction and also the main compounds (Hardwick acid and Colavenic acid) isolated from Copaifera arenicola against S. aureus ATCC 700698 (Methicillin resistant S. aureus) and S. aureus JKD 6008 (Vancomycin-intermediate S. aureus). Regarding antimicrobial assays, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) in microdilution assays were evaluated. The antibiofilm activity, were evaluated by crystal violet (CV) staining to total biomass quantification and count of colony forming units (CFU) in the quantification of the number of viable cells. In general, for the main compounds the MIC and MBC values were 31,25 μg/mL for both strains and for non-volatile fraction values ranged from 15,625 to 125 μg/mL. Regarding antibiofilm activity, in the CFU quantification, was observed a reduction of 2,1 logs to a total decrease in viable cells, compared to the control, with the decrease starting from 15,625 to 1000 μg/mL concentration, for non-volatile fraction and the main compounds for both strains. In the quantification of biomass, was observed significant reductions in the biofilm of both strains, with values between 79 and 100% for non-volatile fraction and the main compounds. In summary, the non-volatile fraction and the major compounds showed significant antimicrobial activity and inhibited biofilm formation. In this way, the compounds isolated of C. arenicola can be an alternative against infections caused by S. aureus biofilms. Palavras-chave: Biofilms, Staphylococcus aureus , Antibacterial, Copaifera arenicola Agência de fomento:CNPq, CAPES e FUNCAP | |||||