| Congresso Brasileiro de Microbiologia 2023 | Resumo: 334-1 | ||||
Resumo:Pseudomonas aeruginosa is a Gram-negative aerobic bacterium adapted to low osmolarity environments and therefore, it is often found in water. P. aeruginosa is considered an opportunistic pathogen due to the wide variety of acute and chronic infections it can cause. In this context, the supply water distribution systems are highlighted because they present multiple favorable factors for the colonization of P. aeruginosa. This study aimed to evaluate the relationship between antibiotic resistance, biofilm formation, virulence factors and source of origin of Pseudomonas aeruginosa environmental isolates with regard to the presence of metallo-β-lactamase-encoding genes. The samples were collected by technicians from the municipal surveillance of the 45 municipalities of Western São Paulo, which carried out a physical-chemical analysis of the water and detection of P. aeruginosa. The isolates were phenotypically characterized by evaluating antimicrobial susceptibility, biofilm formation and motility. In addition, genotypic analysis was performed for the presence of virulence factor genes and antimicrobial resistance genes. The results were statistically analyzed with a significance level of 0.05. A total of 557 water samples were evaluated and of these, 23 were positive for the presence of P. aeruginosa. Approximately 91.7% of the isolates were weak biofilm formers. Only 4 isolates showed antimicrobial resistance. All isolates presented Twitching motility, a positive result for the production of pyocyanin, alkaline protease, and hemolysins. About 65.2% of the isolates presented a positive test for the presence of phospholipases C, while none were positive in the test for the production of lipases. Regarding the genotypic tests it was observed: lasAlasA, (95.6%) lasB (95.6%), exoS (95.6%), exo (91.3%), toxA (91.3%), akgO (91.3%), plcN (91.3%) aprA (86.9%), phzM (78.3%), and pvdA (60.9%). For genes encoding metallo-beta-lactamase, there was the presence of blaVIM (56.6%), blaSPM (4.3%), and blaSIM(47.8%). A strong association was found between the metallo-beta-lactamase producing genes and nine genes related to the production of virulence factors and the motility of P. aeruginosa (r=0.6231). The very close clonal profile suggests a probable similarity between the isolates from some niches in different cities. It can be concluded that the water supply may be contaminated by P. aeruginosa with variable virulence capacities can generate a huge concern for human health. The presence of metallo-beta-lactamase producing genes demands attention because it is easily spread and it can be associated to nine virulence factors genes and the motility of P. aeruginosa. Palavras-chave: Biofilm, ERIC-PCR, Multidrug resistance, Motility, Virulence Agência de fomento:São Paulo Research Foundation - FAPESP | |||||