| Congresso Brasileiro de Microbiologia 2023 | Resumo: 330-1 | ||||
Resumo:In recent years the study of bioactivity compounds immobilization in polymeric matrices to control their release has increased. Bacterial cellulose (BC) is a natural polymer with excellent properties, low cost and sustainable production and has been applied as a base for cosmetic products due to these characteristics. The BC enhances the antibacterial, enzymatic and antioxidant properties of the molecules. Furthermore, BC shows the creation of a delivery system that provides protection to the bioactive compounds, increasing their stability. For this reason the aim of this study was to produce bacterial cellulose as a bioactive compounds carrier, nisin and mandelic acid, to improve the antimicrobial activity. For this reason, the BC was produced through the fermentation of Gluconacetobacter xylinus. After 4 days the BC membranes, with a diameter of 2 cm, as an adhesive, were collected and bioactives were incorporated by immersion for 4h at 25ºC. After this time, the BC were collected and then the antimicrobial activity was evaluated by the agar diffusion assay against Staphylococcus aureus. Posteriorly, nisin (NI) and mandelic acid (MA) solution were analyzed distinctly by Minimum Inhibitory Concentration (MIC) against S. aureus. It was possible to observe that MIC for NI 1.5 mg/mL and for MA was 3.1 mg/mL, however, when NI and MA were mixed, the MIC results did not get better. For the diffusion agar assay was possible to observe that the BC potentiated the antimicrobial activities significantly. When MA was analyzed alone it had 27,34 AU/mL, but when incorporated in BC it has an improvement 5000 times and resulted in 150208,67 AU/mL. NI showed the same behavior as MA, when alone had 239 AU/mL and when incorporated on BC improved 10 times and MA + NI + BC resulted in 215050 AU/mL, being 2200 times higher than just MA + NI. Therefore, the results exhibit that the combination of the actives with bacterial cellulose provide a synergistic action, accordingly, in the near future, our research group aims to develop a successful product for the treatment of bacterial acne with natural properties and low cost. Palavras-chave: Bacterial cellulose, Delivery System, Natural actives Agência de fomento:Coordination for the Improvement of Higher Education Personnel. PROBIC/UNISO | |||||