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Congresso Brasileiro de Microbiologia 2023
Resumo: 325-1

325-1

PRACTICAL RESEARCH AND TEACHING ACTIVITIES IN MICROBIOLOGY: FOCUS ON PATHOGENICITY OF ENTEROINVASIVE ESCHERICHIA COLI (EIEC)

Autores:
Lara Nardi Baroni (ICB - INSTITUTO DE CIÊNCIAS BIOMÉDICAS) ; Bárbara Rodrigues Cintra Armellini (ICB - INSTITUTO DE CIÊNCIAS BIOMÉDICAS) ; Ana Carolina Ramos Moreno (ICB - INSTITUTO DE CIÊNCIAS BIOMÉDICAS, IBU - INSTITUTO BUTANTAN) ; Rita de Cássia Café Ferreira (ICB - INSTITUTO DE CIÊNCIAS BIOMÉDICAS)

Resumo:
Teaching-learning tools in microbiology-based education might influence undergraduate student engagement with science and research. Attempting to promote an authentic learning opportunity outside the classroom, we proposed a multimodal learning experience for undergraduate students from the Biomedical Sciences and Fundamental Health Sciences courses who attended the bacteriology discipline offered at the Institute of Biomedical Sciences of the University of São Paulo. Using two approaches created by our group, "Adopt a Bacteria" and "Real-Lab-Day", the objective of the present study was to evaluate the impact of laboratory scientific practices, encouraging students to participate in a variety of "hands-on" activities that brought them closer to an academic/scientific life environment to foster engaged learning. In the framework of "Adopt a Bacteria", the students adopted the Escherichia coli bacterium and made posts on Instagram about different topics, such as bacterial physiology, metabolism, pathogenicity, and antimicrobial resistance. In this concern, after analysis of word frequency, we observed that the theme "pathogenicity" stood out in the word clouds. Keeping this concept into consideration, a second practical activity was the "Real-Lab-Day." The primary purpose of this activity was to assess the effectiveness of laboratory activities as an approach to bringing students closer to a scientific living atmosphere. The pathogenicity of Shigella flexneri M90T against Enteroinvasive E. coli (EIEC) was used as an experimental model, and the following experiments were carried out: (i) M90T / EIEC invasion and dissemination assays in human intestinal epithelial cells (Caco-2 cells), (ii) Data acquisition, analysis, and scientific poster elaboration/presentation, (iii) Analysis of undergraduate students and scientific mentors (graduate students and pos-docs) responses to questionnaires about the "Real-Lab-Day" activity. The perceived undergraduate students learning and interest in microbiology research were improved by the Real-Lab-Day experience, and the "Adopt a Bacteria" activity guided students in the pathogenicity theme, reinforcing the concept of "learning to learn". We conclude that the teaching methodology involving social media and practical activities related to pathogenicity positively impacted the formation and engagement of students through microbiology.

Palavras-chave:
 active teaching methodology, EIEC, Escherichia coli, teaching


Agência de fomento:
CAPES