| Congresso Brasileiro de Microbiologia 2023 | Resumo: 321-1 | ||||
Resumo:The Schmallenberg virus, first described in 2011, is an important virus in cattle raising, as it can lead to miscarriages and stillbirths. This virus has yet to be described in Brazil. The main form of transmission of this virus is through a vector of the genus Culicoides, studies also indicate that there may be vertical transmission in cattle, during the first trimester of pregnancy, another form of transmission is through the importation of semen and embryos that are contaminated with a certain agent. In this study, we carried out molecular tests in bovine abortions, in an attempt to detect the presence of this virus in Brazilian territory. Vertical transmission of SBV in cattle can lead to miscarriages or stillbirths, usually associated with mummification and congenital malformation. The molecular tests were done in abort fetuses, with congenital malformations previously described in the literature caused by the agent were subjected to extraction of genomic material and molecular tests for BHV-1, B. abortus, N. caninum, T. gondii, BVD and Campylobacter sp. were previously made. The test for the detection of the agent in the samples was based on the real-time PCR test (RT-PCR), based on oligonucleotides from the Simbu serogroup, five samples were considered positive and submitted to Sanger sequencing with 94,64%, 94,12%, 91,07%, 94,64%, 96,72%, identity percentage respectfully. Although serological detection was not performed in adult animals in our study, the presence of the viral agent in fetal tissues indicates that transplacental transmission between mother and fetus occurred, indicating its presence in herds. one of the possible causes of the presence of this virus in the Brazilian territory, is exported semen, since 80% of positive samples were from Holstein breed. 4 samples were from the state of Mato Grosso, despite having a hotter and drier climate, than Europe, has a large population of representatives of the genus Culicoides, which are currently the main vectors for Orthobunyaviruses. Despite all the samples used presenting compatible clinical characteristics, the low agent detection, also observed in other studies, probably were because some of the fetuses age, out of the ideal range of RNA detection. The SBV, despite being an emerging disease that originated in another continent, was confirmed through this work, using macroscopic findings at necropsy, molecular diagnosis and genetic sequencing, is already circulating in Brazil. Palavras-chave: Abortion, Bovine, Schmallenberg virus, RT-qPCR Agência de fomento:Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) e Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |||||