| Congresso Brasileiro de Microbiologia 2023 | Resumo: 302-1 | ||||
Resumo:The gastrointestinal (GI) tract is a complex system that performs different functions, which include: nutrient absorption, digestion, waste excretion, and regulation of microbiome-host interaction. Several studies suggest the importance of gastrointestinal health and how it plays a significant role in the overall health of individuals. However, it is known that dietary components and some drugs such as indomethacin substantially alter intestinal physiology and modulate the integrity of the intestinal barrier and may cause or aggravate conditions similar to Inflammatory Bowel Diseases. In this context, micronization emerges as a strategy to improve physical properties and consequently absorption of natural products with potential health benefits, such as curcumin. This study evaluated the anti-inflammatory and intestinal microbiota modulation effect of a curcumin-carrying nanoemulsion in an animal model of intestinal damage induced by indomethacin. Nanoemulsions containing curcumin were prepared by spontaneous emulsification method with castor oil, soy lecithin and curcumin in ethanol (≥99.8%, HPLC grade). The nanoemulsion was then characterized by dynamic light scattering (DLS) and zeta potential and the morphology evaluated by scanning electron microscopy (SEM). Its stability was tested under different conditions of pH (2.0; 7.0 and 8.0) and storage temperature (8, 30 and 37˚C) at times 0, 7, 14, 21 and 28 days. In animal experimentation, 36 male mice of the Mus musculus lineage (C57BL/6) were used. The animals were randomly divided into 4 groups of 9 mice for an adaptation step for 7 days, pre-induction for 10 days, followed by intestinal damage induction + treatment for 4 days. Its impact on intestinal inflammation was evaluated based on macroscopic, histopathological and metagenomic analysis.The results were compiled in graphs and tables; statistical analysis was performed considering a significance level of 0.05. The results were compiled in graphs and tables; statistical analysis was performed considering a significance level of 0.05. In the present study, a stable nanoemulsion with a size of 409.8 nm, PDI of 0.132 and zeta potential of -18.8 mV was obtained, however, they lost charge at pH2, showing instability in acidic media (p<0.05). In animal experiments, it was demonstrated that curcumin reduced 50% of inflammation and 100% of lymphoid hyperplasia (p<0.05). The nanoemulsion did not significantly improve intestinal inflammation induced by indomethacin. The metagenomic analysis demonstrated that indomethacin reduced the relative frequency of the phylum Firmicutes (p<0.05). The group treated with curcumin nanoemulsion showed a higher relative abundance of the genus Lactobacillus (p<0.05).In conclusion, the effectiveness of curcumin in reducing intestinal inflammation was confirmed. Although the curcumin nanoemulsion did not have a functional effect on intestinal damage, it was relevant in the modulation of the intestinal microbiota. Thus, further studies are needed to improve the formulation of the nanoemulsion and enable its future application in the treatment of inflammatory bowel disease. Palavras-chave: Bioactives, Histopathology, Metagenomics Agência de fomento:FAPESP # 2022/12823-0 | |||||