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Congresso Brasileiro de Microbiologia 2023
 		Resumo: 301-1

301-1

MOLECULAR CHARACTERIZATION OF VIRULENCE FACTORS AND BETA-LACTAM RESISTANCE GENES IN Klebsiella pneumoniae ISOLATED FROM THE TRACHEAL SECRETION OF PATIENTS AT UNIVERSITY HOSPITAL: BLAoxa GENE IMPACTS PATIENT'S CLINICAL OUTCOME

Autores:
Heitor Munhoz Pereira (UNOESTE - Western University of São Paulo) ; Kimberlly de Oliveira Silva (UNOESTE - Western University of São Paulo) ; Luiz Euribel Prestes Carneiro (UNOESTE - Western University of São Paulo) ; Valéria Cataneli Pereira (UNOESTE - Western University of São Paulo) ; Lizziane Kretli Winkelstroter (UNOESTE - Western University of São Paulo)

Resumo:
K. pneumonia is an encapsulated and non-motile bacterium. Also, it is a common cause of healthcare-associated infections (HAI) and long-term care facilities worldwide. The prevalence of nosocomial infections due to K. pneumoniae was recorded up to 10% and infections caused by K. pneumoniae can involve diverse sites such as: lung, urinary tract, surgical wounds, abdominal cavity, intravascular devices, soft tissue, and blood stream. The aim of this study was to carry out the molecular characterization of virulence factors and β-lactam resistance genes of Klebsiella pneumonia isolated from patients at a university hospital and associated with the clinical outcome of the patients. All 56 isolates originated from tracheal secretions of patients admitted to a university hospital. Confirmation of β-lactam resistance genes and virulence factors was performed through polymerase chain reaction. The phenotypic analysis was carried out by the antimicrobial susceptibility test and biofilm formation. Patients' clinical data were collected from hospital records. Statistical analysis was performed considering a significance level of 0.05. The results demonstrated that death of patients with infection caused by K. pneumonia was related to age, as it was more present in patients over 60 years old, as well as the presence of the blaOXA gene, responsible for the greatest resistance to carbapenems in this group (p<0.05). It was also observed that all patients who presented the wabG gene, or at least one of the four genes related to β-lactamase production resulted in death (p<0.05). Despite the presence of genes related to adhesion and mucoviscosity, the formation of biofilms was considered weak in the vast majority of isolates. This study highlights the interest in the adoption of strict measures to prevent and control the spread and implementation of precautions regarding contact and adequate treatment.

Palavras-chave:
 Biofilm , Death, Hospital discharge, Mucoviscosity


Agência de fomento:
FAPESP #2022/11913-6