| Congresso Brasileiro de Microbiologia 2023 | Resumo: 295-1 | ||||
Resumo:The emergence of multidrug-resistant (MDR) pathogens in recent years poses a major health threat worldwide. During the COVID-19 pandemic, changes in personal behavior, hygiene care and usage of antibiotics may have been contributing to changes in microbiota and MDR emergence, such as methicillin-resistant Staphylococcus (MRS). MRS is a leading cause of nosocomial infections and has been associated with neonatal infections, being the anovaginal colonization of pregnant women the main source of vertical transmission. We determined MRS anovaginal colonization rates among pregnant women attending at a single maternity in Rio de Janeiro, Brazil considering the periods before (January 2019 to March 2020; 521), during (May 2020 to June 2021; 360) the pandemic and after the implementation of COVID-19 immunization protocols and relaxation of non-pharmaceutical interventions in Rio de Janeiro (July 2021 to August 2022; 443). Anovaginal specimens (1324) were streaked onto chromogenic media after a pre-enrichment step and colonies were identified by MALDI-TOF MS. Antimicrobial susceptibility was evaluated according to CLSI. Detection of mecA and SCCmec typing was assessed by PCR. Overall, 563 (42.5%) samples were positive for MRS, including 9 distinct species. S. haemolyticus was the most prevalent (MRSH, 508; 90.2%), followed by S. epidermidis (MRSE, 15; 2.7%), S. aureus (MRSA, 12; 2.1%) and S. saprophyticus (MRSS, 9; 1.6%). MRS anovaginal colonization rates among pregnant women significantly (p< 0.001) increased from 8.6% before to 59.7% during the COVID-19 pandemic and increased again to 68.4% in the third period. In general, SCCmec type V was the most frequent among MRS (54.9%) and a high proportion of isolates (41.9%) could not be typed (NT) due to new combinations of ccr and mecA genes (being the combination ccr type 2, ccr type 5 and mecA class C genes the most common). A total of 219 (44.3%) MRS strains were resistant to at least 3 different classes of antimicrobial agents (MDR). The rate of MDR samples decreased significantly (p< 0.001) from 64.4% in the first period to 43.3% in the second period. The increasing rates of MRS colonization among pregnant women included in the study indicate the need for continuing surveillance of this important group of multidrug-resistant pathogens within maternal and neonatal population and highlight possible effects of the pandemic in the dynamic of bacterial infectious diseases. Palavras-chave: ANTIMICROBIAL RESISTANCE, COVID-19 PANDEMIC, METHICILLIN-RESISTANT Staphylococci, PREGNANT WOMAN, SSCmec TYPING Agęncia de fomento:BactiVac Network; CAPES; CNPq; FAPERJ | |||||