| Congresso Brasileiro de Microbiologia 2023 | Resumo: 292-1 | ||||
Resumo:Staphylococcus aureus is a significant causative agent of numerous opportunistic infections. With the rise in the prevalence of these infections, there is a growing need to explore therapeutic alternatives. Chitosan has an inhibitory action on bacterial growth and phenolic compounds have antibacterial action when they interact with the bacterial surface, acting on the plasmatic membrane. Gallic acid an aromatic trihydroxybenzoic acid containing the phenolic ring and the carboxylic acid functional group, has lipophilic and pro-oxidative properties that contribute to its antimicrobial activity but has bioavailability and pharmacokinetic limitations. Nanotechnology presents a potential solution by enabling the development of antimicrobial agents with reduced toxicity and enhanced bioavailability, thereby potentially improving therapeutic efficacy. In this context, a nanostructured system consisting of zein and chitosan loaded with gallic acid was synthesized using the liquid-liquid dispersion method. To determine their antibacterial activity, microdilution in broth was performed according to the Clinical and Laboratory Standards Institute (CLSI) protocol M07-A10, utilizing the Staphylococcus aureus strain ATCC 25923. The resulting nanoparticles exhibited an average diameter of 145.9±9.4 nm, a polydispersion index of 0.297±0.033, and a zeta potential of +17.5±2.6 mV, from scanning electron microscopy, the nanoparticles presented spherical morphology. The Minimum Inhibitory Concentration (MIC) of gallic acid was determined to be 200 µg/mL, whereas the nanoparticle loaded with gallic acid, at a concentration of 10 µg/mL. The nanostructured system developed showed an inhibitory activity superior to that of gallic acid. In addition, it is a system developed with biodegradable and biocompatible polymers, with low production cost. Thus, it may be a promising system for delivering gallic acid with antibacterial properties.
Keywords: chitosan; flavonoid; antibacterial; nanostructured.
Development Agency: Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Palavras-chave: chitosan, flavonoid, antibacteria, nanoestructured Agência de fomento:Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |||||