| Congresso Brasileiro de Microbiologia 2023 | Resumo: 196-2 | ||||
Resumo:The use of antibiotics for the treatment of bacterial infections can be considered an important achievement for modern medicine. However, antimicrobial resistance has become a serious public health problem, mainly induced when this bacterium acquires mobile genetic elements. The epidemiological importance of the circulation of plasmids that confer antimicrobial resistance led to the need to verify which are the main plasmid incompatibility groups (INC) widespread among M. morganii isolates. Therefore, the present study aimed to evaluate the prevalence of resistance genes to several antimicrobials, in addition to tracking and monitoring the circulation of INC groups in 138 isolates of urinary tract infections (UTI) by M. morganii, of community origin (70) and hospital (68), in the city of Londrina-PR, over a period of 5 years (2016-2020). For this, the M. morganii isolates were submitted to the polymerase chain reaction (PCR) test for the detection of the genes blaCTX-M (1, 2, 8, 9 and 25), TEM, SHV, aac'(6)-lb-cr, sul1, sul2, qnrA, qnrB, qnrS, qnrD and fosA3, in addition to the 18 INC groups (HI1, HI2, I1-Iγ, X, L/M, N, FIA, FIB, W, Y , P, FIC, A/C, T, FIIA , F, K and B/O). Of the hospital isolates, 9 were positive for group M-1, 22 for M-2 and 2 for M-9. Regarding the isolates of community origin, 7 were positive for the M-1 group, 1 isolated for M-2 and 2 for M-9. Regarding the research of resistance genes not related to ESBL, in hospital isolates, we detected the presence of 21 (30.88%) qnrD resistant isolates, 10 (24.39%) isolates showed resistance to sul1 and 30 (73.17 %) to sul2. In isolates of community origin, we detected the presence of the qnrD genes in 23 (32.85%), sul1 in 11 (32.35%), while for the sul2 gene, we observed it in 19 (55.88%) of the isolates. The other genes were not detected in any of the M. morganii studied. Regarding the INC groups, for the hospital isolates, the FIB, I1 and A/C groups were detected in 19 (27.94%), 14 (20.58%) and 2 (2.94%), respectively. While in the community, we revealed the presence of INC FIB in 19 (27.14%), I1 in 12 (17.14%) and FIA in 3 (4.28%). Hospital isolates are approximately 8 times more likely to harbor the blaCTX-M-2 gene compared to community M. morganii isolates. Additionally, the prevalence of sulfonamide resistance genes, particularly the sul2 gene, is significantly higher in the hospital setting compared to the community (OR:2.12, CI: 1.2-3.74), indicating that the primary sulfonamide resistance gene in this Hospital is sul2 gene. Notably, the replicons most present in our study were FIB and I1, being the most prevalent, both in the hospital and in the community. Another important information that should be considered is the presence of the INC I1 group associated with the CTX-M1 group, both in the hospital (OR: 3.48 CI: 0.98,12.39) and in the community (OR: 5.75 CI: 1.74,19.06). In addition, we can also note the relationship of the INC FIB group often associated with the sul2 gene, both in the hospital environment and in the community (p< 0.05). Given this epidemiological screening, the circulation of resistance genes to several antimicrobials in these isolates can lead to difficulties in the treatment of these infections, requiring health surveillance measures and control of their infections. Palavras-chave: Plasmid, Resistance, Epidemiological screening, ITU-CA Agência de fomento:Coordenação de Aperfeiçoamento de Pessoal de Nível Superior- CAPES | |||||