| Congresso Brasileiro de Microbiologia 2023 | Resumo: 83-2 | ||||
Resumo:Lippia origanoides, popularly known as alecrim-pimenta, alecrim-do-nordeste and estrepa-cavalo, is a plant native to Latin America, where its essential oils are used traditionally for throat infections, gastroenterical and topical, besides also being employed for analgesia, sedation, and as an expectorant. The world health organization estimates that more than 80% of the world population uses medicinal plants or herbal medicines to treat their diseases, so the search for natural compounds that can promote the development of new drugs more effective against microbial resistance is of great relevance. For the initial evaluation of the antimicrobial potential of L. origanoides, the agar diffusion method was used, where discs were sensitized with 10µL of essential oil solution in triplicate for each bacterium, with the positive control (respective antibiotic) and negative control (blank). The strains used were Escherichia coli, Staphylococcus aureus, Enterobacter aerogenes, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Enterococcus faecalis, seeded on Müller Hinton Agar. The plates were incubated in a bacteriological oven at 37°C for 18-24 hours. And to determine the minimum inhibitory concentration (MIC), the microdilution method was used in a 96-well plate. The plate was incubated at 37°C for 18-24 hours and after this period an indicator of bacterial presence was added. Finally the plate was placed in an incubator for 2 more hours at 37°C and at the end of this period the reading was taken. In the methods used only E. coli showed sensitivity to L. origanoides, showing a zone of inhibition (mm) of 10.67 ± 0.58. However it had a high inhibition potential in the MIC at dilution of 1 ⁄ 8 for E.coli, S.aureus and the E. faecalis. This result may be related to the majority presence of carvacrol and thymol in its compositional structure; both have antimicrobial properties linked to the presence of the hydroxyl group (OH), which dissolves between bacterial membranes and favors their rupture by increasing their permeability. We can conclude that the essential oil of Lippia origanoides presents a rich source of components with pharmacological potential, due to the expression of antimicrobial activity in both Gram-negative and Gram-positive bacteria. This makes it a great ally in the development of new drugs with broad-spectrum antibacterial activity can provide assets with a lower degree of toxicity and be more efficient.
Palavras-chave: Antimicrobial Action, Essential Oils, Medicinal Plants Agência de fomento:Universidade Federal do Pará | |||||