| Congresso Brasileiro de Microbiologia 2023 | Resumo: 83-1 | ||||
Resumo:Piper divaricatum, popularly known as pau-de-angola, is usually found in tropical and temperate regions with heights of 1-10 meters. It has as its main components methyleugenol and eugenol in its essential oil, giving it great medicinal importance, since there is a need to develop drugs aimed at prospecting natural compounds to fight infections resistant to antimicrobial action. For the initial evaluation of the antimicrobial potential of P. divaricatum, the agar diffusion method was used, the disks were sensitized with 10µL of essential oil solution in triplicate for each bacterium, with the positive control (respective antibiotic), negative control and blank (tween 80). The strains used were Escherichia coli, Staphylococcus aureus, Enterobacter aerogenes, Pseudomonas aeruginosa, and Enterococcus faecalis, seeded on Müller Hinton Agar. The plates were incubated in a bacteriological oven at 37°C for 24 hours. To determine the minimum inhibitory concentration (MIC), the 96-well microdilution method was used. The plate was incubated at 37°C for 24 hours and after this period a bacterial presence indicator was added. Finally, the plate was placed in the oven for 2 more hours at 37°C and at the end of this period the reading was taken. P. divaricatum showed satisfactory activity against Gram-positive and Gram-negative bacterial strains, characterizing a broad spectrum of action. The bacteria with greater susceptibility were E. coli, P. aeruginosa and S. aureus, with inhibition zones of 8.7 mm and 9 mm in the diffusion disk test and MIC of 30.63 mg/ml, 30.63 mg/ml and 61.25 mg/ml respectively. Its broad antimicrobial activity may be related to the presence of eugenol in its composition; eugenol's activity is attributed to its phenolic structure. This structure has mechanisms that damage the cell wall and the bacterial membrane, causing protein and lipid leakage and favoring bacterial lysis. These mechanisms make it a great ally in the development of new drugs with broad-spectrum antibacterial activity, being able to provide more efficient actives with a lower degree of toxicity.
Palavras-chave: Antimicrobial Action, Essential Oils, Medicinal Plants Agência de fomento:Universidade Federal do Pará | |||||