| Congresso Brasileiro de Microbiologia 2023 | Resumo: 44-1 | ||||
Resumo:Mycobacterium tuberculosis H37Rv, M. kansasii, and M. avium are major global public health concerns. M. tuberculosis causes tuberculosis, which affects millions of people worldwide. M. kansasii and M. avium cause infections, especially in immunocompromised individuals. Treating these infections is challenging due to drug resistance and long treatment periods. Therefore, the development of new therapies is crucial. In this context, natural products are promising sources of novel compounds with therapeutic potential. Rottlerin, a compound found in Mallotus philippinensis, is known for its antibacterial and anti-inflammatory properties. These properties suggest that rottlerin, a natural compound, may be a promising alternative for the treatment of mycobacterial infections, including tuberculosis. Therefore, this study aimed to evaluate the anti-mycobacterial and antioxidant potential of rottlerin. For this purpose, assays were conducted to determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the compound against the following strains: M. tuberculosis H37Rv (ATCC 27294 - Mt), M. tuberculosis (clinical isolate - MtCI), M. kansasii (ATCC 12478 - Mk), M. kansasii (clinical isolate - MkCI), M. avium (ATCC 25291 - Ma), and M. avium (clinical isolate - MaCI). Additionally, the antioxidant activity of rottlerin was evaluated through a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, and the activity was expressed based on the effective concentration (EC50) required to reduce the initial concentration of DPPH free radicals by 50%. Rottlerin demonstrated bactericidal action against the MtCI strain (MIC/CBM 48,40 然) as well as the MkCI and MaCI strains (MIC/CBM 96,81 然). For the Mt, Mk, and Ma strains, the action was bacteriostatic with an MIC of 48,40 然 against Mt and Mk, and 96,81 然 against Ma. The CBM values were 96,81 然 against Mt and 193,61 然 against Mk and Ma. The EC50 for rottlerin was determined at a concentration of 109.2 然, indicating a modest antioxidant activity. The results showed that rottlerin exhibited bactericidal and bacteriostatic effects against different Mycobacterium strains. In addition, rottlerin demonstrated a modest antioxidant activity. These findings suggest that rottlerin is a promising alternative treatment for mycobacterial infections, including tuberculosis. Further research is warranted to explore its efficacy and safety in preclinical and clinical studies with the ultimate goal of developing new therapeutic options to combat these microorganisms. Palavras-chave: Antimycobacterial, Antioxidant, Mycobacterium spp., Natural products, Rottlerin Ag瘽cia de fomento: Coordena誽o de Aperfei蔞amento de Pessoal de N癉el Superior (CAPES finance code 001), Conselho Nacional de Desenvolvimento Cient璗ico e Tecnol鏬ico (CNPq grant 307974/2019-7; CNPq Scholarship 150679/2021-1). | |||||