Poster (Painel)
| MH-017 | Carbapenem-Resistant Acinetobacter baumannii Strains Carrying blaOXA-72, blaOXA-58, blaOXA-23 and blaOXA-143 Oxacillinase Genes in Southeastern Brazil | | Autores: | Micheli Medeiros (FCF - USP - Faculdade de Ciências Farmacêuticas - USP) ; Charline Antonio (FCF - USP - Faculdade de Ciências Farmacêuticas - USP) ; Patrícia Neves (FCF - USP - Faculdade de Ciências Farmacêuticas - USP) ; Elsa Mamizuka (FCF - USP - Faculdade de Ciências Farmacêuticas - USP) ; Nilton Lincopan (ICB-II-USP - Instituto de Ciências Biomédicas II - USP) |
Resumo
Background: Resistance to carbapenems in Acinetobacter baumannii (Ab) is a major problem in medical institutions around the world, which has been strongly associated to the production of class D β-lactamases encoded by blaOXA-23-, blaOXA-24/40-, blaOXA-58- or blaOXA-51-type genes and their variants. We report the first isolations of carbapenem-resistant A. baumannii (CRAb) carrying blaOXA-72 and blaOXA-58 genes and the dissemination of CRAb carrying blaOXA-23 and blaOXA-143 genes in Southeastern Brazil. Methods: From 2004 to 2008 we have conducted a regional CRAb surveillance survey in Ssoutheast and Southern Brazilian hospitals. Thirty-six CRAb isolates recovered from different patients, hospitalized in eight medical centers, were screened for carbapenemase production and for the presence of genes encoding MβL- and OXA-type β-lactamases. Flanking sequences of blaOXA-type genes were characterized by PCR. PCR products were confirmed by sequencing and genotyping was performed by ERIC-PCR. Results: The presence of genes encoding oxacillinases was observed in 89% of imipenem-resistant Ab, which also showed co-resistance to meropenem, cefepime, ceftazidime, aztreonam, piperacillin/tazobactam, ciprofloxacin and amikacin. Presence of blaOXA-51 and ISAba-1 genes was confirmed in all isolates. Fifteen isolates carried the ISAba-1/blaOXA-23 gene array, one isolate (obtained from a blood culture in 2004) carried the ISAba-3/blaOXA-58/ISAba-3 gene array, two isolates (recovered from burn wound cultures in 2007) harbored the blaOXA-72 gene, and fourteen isolates harbored the blaOXA-143 gene (Genbank accession no. FJ628170, FJ492877 and FJ969387). ERIC-PCR typing revealed genetic diversity among Ab isolates carrying blaOXA-23 and blaOXA-143 genes. Conclusions: This is the first report of CRAb isolates carrying blaOXA-72 and blaOXA-58 genes in Brazil. In this regard, OXA-72 was first reported from an Ab strain in Thailand in 2004, and so far it has been restricted to Acinetobacter isolates from China, South Korea, Taiwan, Italy and Spain. On the other hand, our results confirm the dissemination of CRAb carrying blaOXA-23 and blaOXA-143 genes in Brazilian hospitals.
Financial support: FAPESP
Palavras-chave: OXA-72, OXA-58, OXA-143, Acinetobacter baumannii, carbapenem-resistant |