Poster (Painel)
| MH-038 | CHARACTERIZATION OF CIPROFLOXACIN RESISTANTS ISOLATES FROM BLOODSTREAM INFECTIONS | | Autores: | Lays Katarina Lima Rocha (UPE - INSTITUTO DE CIÊNCIAS BIOLÓGICAS) ; Anna Carolina Soares Almeida (UPE - INSTITUTO DE CIÊNCIAS BIOLÓGICASUFPE - DEPARTAMENTO DE GENÉTICA) ; Maria Carolina de Albuquerque Wanderley (UFPE - DEPARTAMENTO DE ANTIBIÓTICOSUPE - INSTITUTO DE CIÊNCIAS BIOLÓGICAS) ; Rivaldo Lins dos Santos Neto (UPE - INSTITUTO DE CIÊNCIAS BIOLÓGICAS) ; Marinalda Anselmo Vilela (UPE - INSTITUTO DE CIÊNCIAS BIOLÓGICAS) ; Márcia Maria Camargo de Morais (UPE - INSTITUTO DE CIÊNCIAS BIOLÓGICAS) |
Resumo Quinolones are a class of synthetic antibiotics with a broad spectrum of clinical uses. In the last years, the number of bacterial isolates resistant to quinolones has increased due to the widespread use of this class of antibiotics in both hospital and community-acquired infections. In addition, quinolone-resistant strains can carry additional determinants of resistance, which can hardly difficult the treatments. This work aimed to characterize 12 ciprofloxacin-resistant bacterial isolates obtained from blood cultures of inpatients at a public hospital, in Recife, Brazil. Isolates were collected in the period 2008-2010 and identified by biochemical conventional methods. Antimicrobial susceptibility was determined by disk-diffusion method, according to CLSI (2010). The presence of Intl1 (class 1 integrase), blaKPC, blaTEM and blaCTX genes was investigated by specific PCR. The isolates were identified as Klebsiella pneumoniae (8/12), Escherichia coli (2/12) and Enterobacter spp (2/12). Since Klebsiella spp is primarily responsible for blood stream infections among Enterobacteriaceae, these findings were expected. Susceptibility assays showed that 75% of the isolates were resistant to at least one of the aminoglycosides tested (amikacin and/or gentamicin), a common occurrence in ciprofloxacin-resistant bacteria. Isolates were highly resistant to third and fourth generation cephalosporins (83.3% and 75%, respectively) and aztreonam, as well (75%). Moreover, 25% showed resistance to meropenem. PCR analysis demonstrated that 92% of the isolates harbored ESBL genes and 83% presented Intl1 gene. All those meropenem resistant isolates showed the presence of blaKPC gene. These results draw attention to the increase in the frequency in accumulation of distinct resistance mechanisms by quinolone-resistant isolates. Therefore, it could be suggested that the extensive overuse of this antimicrobial is contributing to selection of quinolone resistant isolates, facilitating their permanence in the hospital environment and the acquisition of other determinants of resistance.
Financial support: CNPq, CAPES and FACEPE.
Palavras-chave: quinolone, resistance, ESBL, KPC |